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      Metabolic Engineering of Fatty Acid and Lipid Biosynthesis in Plant Seeds = 식물 종자 지질 생합성 경로의 대사공학 연구

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      https://www.riss.kr/link?id=T17176338

      • 저자
      • 발행사항

        서울 : 세종대학교 대학원, 2025

      • 학위논문사항

        학위논문(박사) -- 세종대학교 대학원 , 분자생물학과 , 2025. 2

      • 발행연도

        2025

      • 작성언어

        영어

      • 주제어
      • DDC

        581.35 판사항(22)

      • 발행국(도시)

        서울

      • 형태사항

        221p. : 채색삽도 ; 26cm

      • 일반주기명

        세종대학교 논문은 저작권에 의해 보호받습니다.
        식물 종자 지질 생합성 경로의 대사공학 연구
        [열람제한 : 2027년 01월 01일까지 (특허출원 준비)]
        지도교수:Hyun Uk Kim
        참고문헌: p. 172-206

      • UCI식별코드

        I804:11042-200000848009

      • 소장기관
        • 세종대학교 도서관 소장기관정보
      • ※ 해당 논문은 저작자의 요청에 따라 [원문보기]가 제공되지 않습니다.
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      목차 (Table of Contents)

      • ABSTRACT
      • TABLE OF CONTENTS
      • LIST OF TABLES
      • LIST OF FIGURES
      • CHAPTER 1 Applications and prospects of genome editing in plant fatty
      • ABSTRACT
      • TABLE OF CONTENTS
      • LIST OF TABLES
      • LIST OF FIGURES
      • CHAPTER 1 Applications and prospects of genome editing in plant fatty
      • acid and triacylglycerol biosynthesis
      • 1.1. Abstract
      • 1.2. Introduction
      • 1.2.1. CRISPR/Cas9 and lipid metabolic engineering
      • 1.2.2. Mutation in FAD2
      • 1.2.3. Mutation in FATB and KASI
      • 1.2.4. Mutation in FAE1
      • 1.2.5. Mutation in acyltransferases
      • 1.2.6. Mutation in phospholipases
      • 1.2.7. Mutation in TAG lipases
      • 1.2.8. Increase the TAG in vegetative tissue
      • 1.3. Conclusion and future perspective
      • CHAPTER 2 C-to-G base editing enhances oleic acid production by
      • generating novel alleles of FATTY ACID DESATURASE 2 in plants
      • 2.1. Abstract
      • 2.2. Introduction
      • 2.3. Materials and methods
      • 2.3.1. Plant materials and growth conditions
      • 2.3.2. Plasmid construction
      • 2.3.3. Arabidopsis transformation and transgenic plant selection
      • 2.3.4. Sanger sequencing
      • 2.3.5. Fatty acid analysis
      • 2.3.6. Germination rate
      • 2.3.7. Measurement of root growth
      • 2.3.8. Statistical analysis of the data
      • 2.4. Results
      • 2.4.1. Application of the base editors in planta to generate “attenuated” fad2 alleles
      • 2.4.2. Analyses of the base-editing patterns and lipid contents from the fad2 lineages
      • 2.4.3. Isolation of the base-edited fad2 alleles
      • 2.4.4. Characterization of the growth responses of fad2 alleles
      • 2.5. Discussion
      • CHAPTER 3 Modulation of fatty acid elongation enhances hydroxy fatty
      • acid biosynthesis and accumulation in Arabidopsis
      • 3.1. Abstract
      • 3.2. Introduction
      • 3.3. Materials and methods
      • 3.3.1. Plant materials, growth condition, and Arabidopsis transformation
      • 3.3.2. Vector construction of PfKCS18
      • 3.3.3. Fatty acid analysis
      • 3.3.4. Analysis of the seed size, weight, and plant phenotype
      • 3.3.5. Measure of the seed germination rate and the median germination time
      • 3.3.6. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis
      • 3.4. Results
      • 3.4.1. Changes of the fatty acid composition in the pCam5, pCam5+PfKCS18, and
      • pCam5-atfae1+PfKCS18 lines in the T1, T2 and T3 generations
      • 3.4.2. The pCam5-atfae1+PfKCS18 increased the HFA FAME but reduced the
      • total FAME content
      • 3.4.3. Seed phenotype was partially recovered in pCam5+PfKCS18 and pCam5-
      • atfae1+PfKCS18
      • 3.4.4. Seed germination and plant growth phenotype of the transgenic lines
      • 3.4.5. Expression of castor transgenes and Arabidopsis endogenous genes in the transgenic lines
      • 3.5. Discussion
      • CHAPTER 4 Push, pull, and protect strategy for increasing hydroxy fatty acid in Arabidopsis and Camelina sativa
      • 4.1. Abstract
      • 4.2. Introduction
      • 4.3. Materials and methods
      • 4.3.1. Arabidopsis materials, growth condition, and transformation
      • 4.3.2. Camelina materials, growth condition, and transformation
      • 4.3.3. Vector construction of castor genes to increase the HFA in Arabidopsis
      • 4.3.4. Vector construction of castor genes to increase the HFA in camelina
      • 4.3.5. Fatty acid analysis
      • 4.3.6. Analysis of the seed size and weight in plants
      • 4.3.7. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
      • analysis
      • 4.3.8. Statistical analysis
      • 4.4. Results
      • 4.4.1. Test of the push, pull, and protect strategy in accumulating HFA transgenic Arabidopsis
      • 4.4.2. Seed size and dry weight-based content of the seed oil in single gene transgenic plants
      • 4.4.3. Transgene and FAE1 expression in transgenic plants confirmed by RT-qPCR
      • 4.4.4. Overexpression of multiple RcGPAT9, RcOLE2, and RcLPAT2 does not enhance the HFA content in the pCam5-atfae1-RcWRI1 line
      • 4.4.5. Applying the strategy of enhancing HFAs by expressing five castor genes, which is proven in Arabidopsis, to the oil crop Camelina sativa
      • 4.4.6. Additional introduction of castor genes into CspCam5 camelina for increasing HFAs
      • 4.4.7. Seed size, weight, and dry weight-based content of the seed oil of transgenic camelina
      • 4.5. Discussions
      • REFERENCES
      • 국문초록
      • CURRICULUM VITAE
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