별갑이 아토피 피부염에서의 알러지성 염증 반응에 미치는 영향 = Effects of Amyda sinensis on Allergic Inflammation Mechanism related Atopy Dermatitis

다국어 초록 (Multilingual Abstract)

Objectives: This study aimed to investigate the effects of Amyda sinensis (AS) on allergic inflammation mechanism related atopy dermatitis. Methods: To investigate the effects of AS, We study inhibitory effect of AS on the levels of pro-inflammatory cytokines released from Raw264.7 cell stimulated with LPS (lipopolysaccaride), and EoL-1, THP-1, Jutkat cell stimulated with DP (Dermatophagoides pteronyssinus), and LPS indused acute inflammatory BALB/c mouse model. Result: AS reduced the levels of IL-1β released from Raw264.7 cell stimulated with LPS at 20 ug/ml, 5 ug/ml concentration, and reduced the levels of IL-6 in a dose-dependent. AS significantly reduced the levels of MCP-1 released from EoL-1 cell stimulated with DP (Dermatophagoides pteronyssinus) at all the concentration, and significantly reduced the level of IL-8 at 0.1 ug/ml concentration. AS significantly reduced the levels of MCP-1 released from THP-1 cell stimulated with DP (Dermatophagoides pteronyssinus) at 1 ug/ml concentration, and reduced the level of IL-6 in a dose-dependent. AS significantly reduced the levels of IL-4 released from Jutkat cell stimulated with DP at all the concentration, and significantly reduced the level of IL-5 at 0.1 ug/ml. 1 ug/ml concentration,. and reduced the level of TNF-α in a dose-dependent. AS significantly reduced the levels of TNF-α, IL-6, IL-1β, in LPS indused acute inflammatory BALB/c mouse model, in a dose-dependent. Conclusion: These result suggested that AS has suppressive effect on pro-inflammatory cytokines in various cell lines through the regulation of immune system. AS could be applied on the medicinal sources for treatment of immune abnormal diseases such as atopy dermatitis afterward.

참고문헌 (Reference)

1 박두병, "凉血除濕湯이 DNCB로 유도된 NC/Nga mice의 아토피 피부염에 미치는 영향" 대한한방소아과학회 23 (23): 263-291, 2009

2 피선명, "피부병중의치료여방제" 과학기술문헌출판사 45-58, 1997

3 은희철, "피부과학(5판)" 여문각 95-174, 2008

4 정승일, "천연물 유래 Th2 케모카인 억제제 발굴에 의한 새로운 아토피 피부염 치료기술 개발 : 아토피 피부염 모델 NC/Nga 마우스에서 고삼 추출액의 억제 효과" 대한한의학방제학회 17 (17): 141-151, 2009

5 가민, "중의피부과임상실천" 귀주과학출판사 202-, 2002

6 범서강, "중서의결합치료피부병성병" 광동 인민출판사 188-, 2005

7 서삼문, "중국피부병비방전서" 과학기술문헌출판사 333-336, 2003

8 장지례, "장지례피부병임상경험집요" 중국 의약과기출판사 247-251, 2002

9 노석선, "원색피부과학" (주)아이비씨기획 499-501, 2006

10 강란이, "아토피피부염 동물 병태모델에서의 형개연교 탕의 면역조절작용" 18 (18): 47-62, 2009

11 안종현, "선퇴가 인간의 THP-1 단핵구에서 사이토카인 유전자 발현에 미치는 영향" 대한한방안이비인후피부과학회 23 (23): 94-110, 2010

12 전국한의과대학 본초학교수, "본초학" 영림사 601-603, 1991

13 염정호, "니켈 및 코발트의 세포독성 기전에서 NO의 역할" 13 (13): 274-277, 2001

14 안상현, "금은화의 NF-κB 활성 억제를 통한 iNOS 조절이 NC/Nga 생쥐의 아토피 피부염에 미치는 영향" 대한동의병리학회 24 (24): 278-283, 2010

15 Singh VK, "The paradigm of Th1 and Th2 cytokines: its relevance to autoimmunity and allergy" 20 (20): 147-161, 1990

16 Auwerx J, "The human leukemia cell line, THP-1: a multifaceted model for the study of monocyte - macrophage differentiation" 47 (47): 22-31, 1991

17 Matsukawa A, "Production of IL-1 and IL-1 receptor antagonist and the pathological significance in lipopolysaccharide-induced arthritis" 93 (93): 206-211, 1993

18 Sullivan TJ, "Possible role of arachidonic acid and its metabolites in mediator release from rat mast cells" 122 (122): 431-436, 1979

19 Bomalaskim JS, "Phospholipase A2-activating protein induces the synthesis of IL-1 and TNF in human monocyte" 154 (154): 4027-4031, 1995

20 Auwex J, "Leuk Res" 16 (16): 317-327, 1992

21 Novak N, "Immune mechanisms leading to atopic dermatitis" 112 (112): 128-139, 2003

22 Noyori K, "Experimental induction of arthritis in rats immunized with Escherichia coli 0:14 lipopolysaccharide" 21 (21): 484-488, 1994

23 Mayumi M, "EoL-1, a human eosinophilic cell line" Leuk Lymphoma 243-250, 1992

24 Fry L, "Ellis CN.An Atlas of Aropic Eczema.Informa Healthcare" 87-101, 2003

25 Wong CK, "Differentiation of a human eosinophilic leukemic cell line, EoL-1: characterization by the expression of cytokine receptors, adhesion molecules, CD95 and eosinophilic cationic protein (ECP)" 68 (68): 317-323, 1999

26 Hamid Q, "Differential in situ cytokine gene expression in acute versus chronic atopic dermatitis" 94 (94): 870-876, 1994

27 Ono SJ, "Chemokines: roles in leukocyte development, trafficking, and effector function" 111 (111): 1185-1199, 2003

28 Blaser K, "Cell Regulation in Allergy, Asthma and Atopic Skin Diseases" S Karger Pub 121-137, 2008

29 김병훈, "CD29 및 CD98 활성 매개에 의한 Jurkat T 세포의 유착과 그 활용" 대한약학회 53 (53): 119-124, 2009

30 Meagher LJ, "Atopic dermatitis; review of immunopathogenesis and advances in immunosuppressive therapy" 43 (43): 247-254, 2002