알코올 전처치한 흰쥐에 Cyclohexane 투여로 인한 간 세포 내 소포체 미세구조의 변화 = Ultrastructural Changes of Endoplasmic Reticulum on Hepatocytes by Cyclohexane Injection in Alcohol-pretreated Rats

다국어 초록 (Multilingual Abstract)

To evaluate the effects of ingestion of alcoholic drinks on the toxicities of industrial compounds, cyclohexane (CH) was intraperitoneally administrated to rats (1.56g/kg body weight), which had been ingested 15% ethanol for up to 6 weeks,4 times by once a day and every other day. Following the last treatment of ethanol or CH, blood and liver tissues were collected after 4 hours prior to sacrifice of animals. By the injection of CH, liver weight (% of body weight) and xanthine oxidase activity in serum were increased, and glucose-6-phasphatase (G6P) activity in liver was decreased compared to them of control group. The activities of CH metabolizing enzymes, such as cytochrome P450 dependent aniline hydroxylase (CYPdAH) and alcohol dehydrogenase (ADH), were significantly increased by injection of CH, and those activities were the highest in CH-injected group after pretreated with alcohol. Ultrastructurally. both of alcohol treatment and CH injection induced transforming into the smooth-endoplasmic reticulum from rough-endoplasmic reticulum, the those rate was the highest in case of CH-injection after pretreated with alcohol. From these results, it is suggested that alcohol intake on a level without alcoholic degeneration of hepatocytes could enhance the CH metabolism of liver.

참고문헌 (Reference)

1 "p-nitroanisole reversal of aceton enhanced aniline hydroxylation" 311-317, 1982

2 "metabolic and toxic effects of ethanol" 573-592, 1991update1991

3 "The pharmacokinetics and macromolecular interactions of trichloroethylene in mice and rats" 62 : 137-152, 1982

4 "Methods of enzymatic analysis" Academic Press 1974

5 "Lung uptake and metabolism of cyclohexane in shoe factory workers" 45 : 261-269, 1980

6 "Lung injury after hepatoenteric ischemia-reperfusion role of xanthine oxidase" 154 : 1364-1369, 1996

7 "Injury by drugs and toxins Electron Microscopy in Human Disease edited by J" McGraw-Hill 89-116, 1979

8 "Influence of ethanol or albumin metabolism edited by M" Plenum Press 79-100, 1977

9 "Increased carbon tetrachloride hepatotoxicity and its metabolism after chronic ethanol consumption" 415-66 422, 1974

10 "Implication of free radical mechanisms in ethanol-induced cellular injury" 12 : 219-240, 1992

11 "Exposure to organic solvents-I A survey on the use of solvents The Annuals of Occupational Hygiene 34" 371-378, 1990

12 "Exposure monitoring and health effect studies of workers occupationally exposed to cyclohexane vapor" 65 : 343-350, 1994

13 "Evaluation of the subacute nephrotoxicity of cyclohexane and other industrial solvents in the female Sprague-Dawley rat" 45 : 271-280, 1989

14 "Ethanol metabolism, cirrhosis and alcoholism" 257 (257): 59-84, 1997

15 "Ethanol enhancement of cocaine-induced hepatotoxicity" 30 : 453-458, 1980

16 "Effects of ethanol on the kinetics of methyl ethyl ketone in man" 325-330, 1990

17 "Effect of cyclohexane treatment on the liver cytochrome P-450 dependent aniline hydroxylase activity in alcohol-pretreated rats" 29 : 23-28, 2003

18 "Effect of chronic ethanol administration in bromobenzene liver toxicity in the rat" 66-77, 1983

19 "Dose related effects of single dose of ethanol on the metabolism in rat liver of some aromatic and chlorinated hydrocarbons" 60 : 8-15, 1981

20 "Diminished rates of glucuronidation and sulation in perfused rat liver after chronic ethanol administration" 35 : 439-447, 1986

21 "Cytochrome P450-related differences between rats and mice in the metabolism of benzene toluene and trichloroethylene in liver microsomes" Elovaara E 1079-1085, 1993

22 "Absorption and alveolar excretion of cyclohexane in workers in a shoe factory" 220-223, 1981

23 "A modified colormetric assay for xanthine oxidase in rat liver extracts" 295-308, 1984

24 "A kinetic photometric method for serum gamma-glutamyl transpeptidase" 15 : 124-136, 1969

25 "A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase" 28 : 58-63, 1957