Genetic Screening of the Dazl-Interacting Protein Genes

다국어 초록 (Multilingual Abstract)

have been observed frequently in male infertility patients,suggesting that genes in these regions are involved in malegerm cell development. DAZ is a representative male infertilitygene at the AZFc locus of the Y chromosome. Since DAZcontains an RNA binding motif along with so-called a DAZdomain, it was proposed to participate in RNA metabolismduring spermatogenesis. A mouse gene homologous to thehuman DAZ gene has been cloned and named Dazl (DAZ-like). Dazlat a low level in the ovary. Male mice homozygous for theDazl null allele have small testes with a few spermatogoniaand almost complete absence of germ cells beyond thespermatogonial stage, suggesting the requirement of Dazlfor entry or progression through meiosis. However, its exactcellular functions have not been understood yet. In order toinvestigate cellular functions of Dazl, we decided to isolatecandidate interacting protein genes of the mouse Dazl, usingyeast two-hybrid screening. A number of candidate Dazl-Hgs, Murr1, Nbak3 and Ranbp9, but dynein light chain 1(Dlc1) was most predominant. A strong interaction of Dazlwith Dlc1 suggests that Dazl might function as an mRNAadaptor to the dynein motor complex.

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