황금으로부터 항산화 활성 성분의 분리

국문 초록 (Abstract)

황금 (Scutellaria baicalensis Gergi)에 함유된 항산화 억제활성 물질을 분리하고 항산화 효과에 대하여 조사한 결과는 다음과 같다. 1. 황금 500g을 100% MeOH로 추출하여 150g의 수율을 얻었으며, 다시 분획하여 Ethyl ether 13.2g과 n-BuOH 10.3g, Water 126.5g을 얻었다. 2. Ethyl ether 분획을 Column chromatography, TLC 및 HPLC를 이용하여 xanthine oxidase 억제활성을 가진 compound I을 분리하였다. 3. 1H-NMR, 13C-NMR, MS spectrometer 등 분광학적 방법을 이용하여 Compound I의 구조를 규명한 결과, flavonoid 물질로서 compound I은 구조식이 3,5,7-trihydroxy-2'-methoxy-flavanone로 확인되었다.

다국어 초록 (Multilingual Abstract)

Root of Scutellaria baicalensis G. was extracted with methanol and water to give the yield of 30.0% in order to find the antioxidant substance. The extract was fractionated with diethyl ether, n-butanol and water to test the inhibitive activity against xanthine oxidase. Three fractions inhibited the activities of xanthine oxidase by 48.2%, 10.2% and 2.8%, respectively, at the amount of 0.1 μg. A component that exhibited strong inhibition of xanthine oxidase was isolated from diethyl ether fraction (SE Fr.) by silica gel column chromatography and HPLC, and then identified by 1H-NMR, 13C-NMR and MS spectrophotometry. EDA (Electron Donating ability) of the compound was 28.5% at the concentration 100 μg/3 ml. That was identified to be 3,5,7-trihydroxy-2'-methoxyflavanone by spectrophotometric analysis using 1H-NMR, 13C-NMR and Mass spectrophotometry.