The Comparison of Enzymatic Degradation of Acetylated Xylan by Two Xylanases from P hanerochaete chrysosporium = The Comparison of Enzymatic Degradation of Acetylated Xylan by Two Xylanases from P hanerochaete chrysosporium

다국어 초록 (Multilingual Abstract)

Xylan is the major hemicellulose. The main chain is consisted of xylose residues, whereas the side chain differs by the plant species. For instance, xylan from hardwood is substituted by glucuronic acid and acetylated. In nature, fungi degrade xylan, producing various enzymes.
In general, xylan main chain is degraded into xylooligosaccharides by xylanase. Thus, its substrate recognition is a crucial for the efficient digestion of xylan. Ample studies have reported about xyalanases’ substrate specificities towards xylan and xylooligosaccharides substituted with glucuronic acid. On the other hand, little is known about the effect of acetylation due to difficulty in extracting acetylated xylan.
The basidiomycetes Phanerochaete chrysosporioum is a model organism for white rot fungi. P.c degrades hardwood in nature and has various enzymes. P.c has three xylanases belonging to GH family 10 and 11. In general, wood decay fungi have GH family 10 xylanases and mold has GH family 11 xylanases.
Therefore, this study prepared substrates, investigated the reaction property of two xylanases from the P.c and compared GH family 10 and 11 xylanases’ characteristics. Finally, we proposed xylan degradation system using each xylanase.