환수율 및 접종밀도에 따른 초소형 rotifer, Synchaeta kitina의 대량배양 = Mass Culture of Ultra-small Rotifer, Synchaeta kitina at the Exchange Rate of Culture Water and Initial Inoculation Density

다국어 초록 (Multilingual Abstract)

The productivity of ultra-small rotifer, Synchaeta kitina was investigated at the exchange rate of culture water (10, 20, 30, 40 and 50%) and initial inoculation densities (250, 600 and 900 inds. per mL) in semi-continuous culture. Also, the possibility of mass culture was investigated in a 100 L culture tank. Tetraselmis suecica was used as the feed for S. kitina in all experiments. The production of S. kitina increased with an increase in exchange rate of culture water. The highest production (82.0×105 inds.) was achieved at 40% exchange rate of culture water. The production of S. kitina increased with an increase of initial inoculation density during the first week and the highest total production (17.4×106 inds.) was achieved at 900 inds. per mL of initial inoculation density. However, on the second week, all treatments were not significantly different in total production (P>0.05). During the two weeks, total production of S. kitina at 900 inds. per mL of initial inoculation density was higher than at 600 inds. of initial inoculation density, but there was no significant difference (P>0.05). In the 100 L culture tank, density of S. kitina was kept from 516 to 890 inds. per mL and S. kitina was daily harvested 15.5×106 to 26.7×106 during the experimental period. The production cost for 100 million rotifers in semi-continuous culture was 63,656 won. The results from this study indicate that the optimal exchange rate of culture water and initial inoculation density for the semi-continuous culture of ultra-small rotifer, S. kitina are 40% and 600 inds. per mL, respectively.

참고문헌 (Reference)

1 박진철, "초소형 Rotifer Synchaeta kitina의 성장을 위한최적 염분 및 수온 조건" 한국양식학회 21 (21): 70-75, 2008

2 박진철, "초소형 Rotifer Synchaeta kitina의 성장을 위한최적 먹이 및 공급량" 한국양식학회 21 (21): 76-81, 2008

3 Kohno, H, "Why is grouper larval rearing difficult?: an approach from the development of the feeding apparatus in early stage larvae of the grouper" 44 : 267-274, 1997

4 Schlüter, M, "The inhibition by ammonia of population growth of the rotifer, Brachionus rubens, in continuous culture" 46 : 215-220, 1985

5 Suchar, V.A, "The effects of algae species and densities on the population growth of the marine rotifer, Colurella dicentra" 337 : 96-102, 2006

6 Yu, J, "The effect of un-ionized ammonia on the population growth of the rotifer in mass culture" 52 : 1509-1513, 1986

7 SPSS Inc., "SPSS Base 14.0 for windows"

8 Shiel, R.J, "Rotifera from australian inland waters. Ⅸ. Gastropodidae, Synchaetidae, Asplanchnidae (Rotifera: Monogononta)" 117 : 111-139, 1993

9 Yoshimura, K, "Recent development of a high density mass culture system for the rotifer Brachionus rotundiformis Tschugunoff" 358 : 139-144, 1997

10 Lee, K.W, "Productivity of freshwater rotifer, Brachionus calyciflorus and marine rotifer, B. rotundiformis in the semi-conti- nuous high density culture" 34 : 156-159, 2001

11 Boraas,M.E, "Population dynamics of food-limited rotifers in two-stage chemostat culture" 28 : 546-563, 1983

12 Ooms-Wilms, A.L, "Ooms-Wilms, A.L., G. Postema and R.D. Gulati. 1999. Population dynamics of planktonic rotifers in Lake Loosdrecht (The Netherlands) in relation to their potential food and predators" 42 : 77-97, 1999

13 Duncan,D.B, "Multiple-range and multiple F tests" 11 : 1-42, 1955

14 Park,J.C, "Mass culture of rotifer, Synchaeta sp" Kangnung-Wonju National University 2007

15 Assavaaree, M, "Low-temperature preservation (at 4°C) of marine rotifer Brachionus" 32 : 29-39, 2001

16 Duray, M.N, "Larval rearing of the grouper Epinephelus suillus under laboratory conditions" 150 : 63-76, 1997

17 Park, H.G, "High density culture of the freshwater rotifer, Brachionus calyciflorus" 446/447 : 369-374, 2001

18 Park, H.G, "Growth of rotifer by the air, oxygen gas-supplied and the pH-adjusted and productivity of the high density culture" 32 : 753-757, 1999

19 Yoshimura, K, "Factors inhibiting growth of the rotifer Brachionus plicatilis in high density cultivation by feeding condensed Chlorella" 60 : 207-213, 1994

20 Assavaaree, M, "Factors affecting low temperature preservation of the marine rotifer Brachionus rotundiformis Tschugunoff" 446/447 : 355-361, 2001

21 Lubzens, E, "Biotechnology and aquaculture of rotifers" 446/447 : 337-353, 2001

22 Dhert, P, "Advancement of rotifer culture and manipula- tion techniques in Europe" 200 : 129-146, 2001

23 Yoshimura, K, "A novel culture system for the ultra-high-density production of the rotifer, Brachionus rotundiformis-a preliminary report" 227 : 165-172, 2003