국산 한약재로 이용되는 약용식물의 NCI-H1229 인간 폐암 세포주에 대한 성장 억제효과 분석

다국어 초록 (Multilingual Abstract)

Lung cancer, the most common malignant disease worldwide, is the predominant cause of cancer deaths, particularly amongst men. Therefore, various researchers have focused on the growth inhibitory effects of medicinal plants used in traditional Korean medicine. This study aimed to investigate the growth inhibitory effects of ethanol extracts of Rubiae radix, Inulae flos, Nelumbinis receptaculum, Astilbe radix, and Lagerstroemia flos on NCI-H1229 cells. Method and Results: The viability of NCI-H1229 cells was evaluated in vitro using an MTS assay. Treatment with the ethanol extracts of the selected medicinal plants at 500 ㎍/㎖ reduced NCI-H1229 cell viability and increased apoptotic cell death and caspase-3 activation. In addition, treatment with ethanol extracts of Inulae flos and Astilbe radix increases DNA fragmentation, as measured by the TUNEL assay. Conclusions: These results indicated that ethanol extracts of Rubiae radix, Inulae flos, Nelumbinis receptaculum, Astilbe radix, and Lagerstroemia flos exhibited growth inhibitory effects, inducing apoptotic cell death, DNA fragmentation and caspase-3 activation in NCI-H1229 cells. Therefore, these medicinal plant extracts may be used in the development of natural medicines to inhibit the growth of lung cancers. However, further study is needed to determine the active ingredients of the ethanol extracts from medicinal plants that are reposible for the inhibitory effect on lung cancer cell grwoth.

목차 (Table of Contents)

• ABSTRACT
• 서 언
• 재료 및 방법
• 1. 실험재료와 추출물의 제조
• 2. 세포 배양
• 3. MTS Assay
• 4. Caspase-3 활성 측정
• 5. 세포분석기 (flow cytometry)를 이용한 암세포 사멸 효과측정
• 6. 통계처리
• 결과 및 고찰
• 1. 약용식물 에탄올 추출물이 NCI-H1229 세포생존율에미치는 영향
• 2. 약용식물 에탄올 추출물이 caspase-3 단백질 활성화에 미치는 영향
• 3. 약용식물 에탄올 추출물의 세포사멸에 미치는 영향
• 4. 약용식물 에탄올 추출물의 DNA 단편화에 미치는 영향
• 감사의 글
• REFERENCE

참고문헌 (Reference)

1 엄지나, "황백추출물 및 분획물의 Nitric Oxide 생성 억제 효과 및 Elastase 억제 효과" 한국약용작물학회 25 (25): 22-28, 2017

2 이영주, "폐암의 조기 진단과 치료의 최신지견" 대한내과학회 80 (80): 529-531, 2011

3 성경은, "폐암 환자의 항우울제 처방현황과 관련 요인 분석" 한국임상약학회 26 (26): 107-114, 2016

4 김대현, "천초근의 발암성 연구를 위한 분석 및 안정성 시험" 한국생약학회 43 (43): 147-151, 2012

5 노숙령, "인체 폐암세포주 NCI-H1299에 대한 당근 추출물의 항암효과" 동아시아식생활학회 12 (12): 289-298, 2002

6 김경석, "옻나무추출물 위주의 한방치료로 국소 진행된 비소세포폐암 환자의 잔존 종양의 관해와 생존기간이 연장된 사례" 대한한방내과학회 36 (36): 200-206, 2015

7 원범영, "에탄올 농도에 따른 미나리 추출물의 영양성분 변화" 한국식품영양과학회 44 (44): 882-887, 2015

8 배지현, "식중독유발 세균의 증식에 미치는 천초근 추출물의 영향" 한국식품영양과학회 34 (34): 389-394, 2005

9 김금숙, "식물자원의 Prolyl Endopeptidase 저해활성 탐색" 한국약용작물학회 12 (12): 1-9, 2004

10 윤정식, "식물자원으로부터 Angiotensin converting enzyme 저해활성 탐색" 한국약용작물학회 11 (11): 246-251, 2003

11 노종현, "선복화 에탄올 추출물의 Nitric Oxide 생성, 산화스트레스 및 대장암 세포 억제효과" 한국약용작물학회 26 (26): 19-25, 2018

12 고현민, "서양금혼초 부위별 추출물의 티로신에이즈, α-글루코시다아제 저해활성 및 항산화효과" 한국약용작물학회 25 (25): 139-145, 2017

13 우경완, "배롱나무의 항산화 활성 성분" 한국생약학회 47 (47): 204-210, 2016

14 김아현, "노루오줌으로부터 Bergenin의 함량 분석 및 에탄올 추출물의자궁경부암세포 성장 억제 효과" 한국생약학회 49 (49): 47-54, 2018

15 노종현, "노루오줌 에탄올 추출물의 산화스트레스 및 대장암 세포 억제활성" 한국약용작물학회 26 (26): 141-147, 2018

16 최호승, "茜草根 추출물이 HL-60 세포주의 세포자멸사 기전에 미치는 영향" 한의병리학회 22 (22): 548-555, 2008

17 Zhao L, "The fanconi anemia pathway sensitizes to DNA alkylating agents by inducing JNK-p53-dependent mitochondrial apoptosis in breast cancer cells" 45 : 129-138, 2014

18 Xu YM, "Tannins and related compounds. CVII. structure elucidation of three new monomeric and dimeric ellagitannins, flosin B and reginins C and D, isolated from Lagerstroemia flos-reginae RETZ" 39 : 647-650, 1991

19 Amashukeli X, "Subcritical water extraction of amino acids from atacama desert soils" 112 : G04S16-, 2007

20 Sun HX, "Studies on the chemical constituents in radix Astilbes chinensis" 27 : 751-754, 2002

21 Yuan J, "Roles of caspases in necrotic cell death" 167 : 1693-1704, 2016

22 Kim JH, "Role of antiapoptotic proteins in tumor necrosis factor-related apoptosisinducing ligand and cisplatin-augmented apoptosis" 9 : 3134-3141, 2003

23 Hyun-TaiLee, "Pseudoguaianolides Isolated from Inula britannica var. chinensis as Inhibitory Constituents against InducibleNitric Oxide Synthase" 대한약학회 25 (25): 151-153, 2002

24 Rieger AM, "Modified annexin V/propidium iodide apoptosis assay for accurate assessment of cell death" 50 : 2597-, 2011

25 Chou SM, "Involvement of p38 MAPK in the anticancer activity of cultivated Cordyceps militaris" 43 : 1043-1057, 2015

26 Choi YH, "Induction of apoptosis and telomerase activity of NCI-H460 human lung carcinoma cells by methanol extract of Houttuynia cordata thunberg" 17 : 364-371, 2012

27 Sarna L, "Impact of respiratory symptoms and pulmonary function on quality of life of ling-term survivors of non-small cell lung cancer" 125 : 439-445, 2004

28 Kang BH, "Illustrated book of ecology in Korean herbal medicine" Geobook 487-, 2008

29 Fu T, "Hyperoside induces both autophagy and apoptosis in non-small cell lung cancer cells in vitro" 37 : 505-518, 2016

30 Alnemri ES, "Human ICE/CED-3 protease nomenclature" 87 : 171-, 1996

31 이세희, "HT-29 대장암 세포에서 식물추출물 쿼세틴에 의한 Sestrin2의 조절과 Apoptosis 유도에 관한 연구" 대한암예방학회 17 (17): 244-250, 2012

32 박찬흠, "H2O2로 유도된 C6 신경교세포의 세포사멸에 대한 감초 추출물과 감초 활성물질의 보호효과" 한국약용작물학회 25 (25): 315-321, 2017

33 이은경, "Flowers of Inula japonica Attenuate Inflammatory Responses" 대한면역학회 10 (10): 145-152, 2010

34 Belloc F, "Flow cytometry detection of caspase 3 activation in preapoptotic leukemic cells" 40 : 151-160, 2000

35 Thomas E, "Extract of Vernonia condensata, inhibits tumor progression and improves survival of tumorallograft bearing mouse" 6 : 23255-, 2016

36 Kim MJ, "Evaluation of antioxidative effect from Lotus seed and seedpod extracts" Dongguk University 2013

37 Wu M, "Essential oils from Inula japonica and Angelicae dahuricae enhance sensitivity of MCF-7/ADR breast cancer cells to doxorubicin via multiple mechanisms" 180 : 18-27, 2016

38 Ismail T, "Ellagitannins in cancer chemoprevention and therapy" 13 : E151-, 2016

39 오창모, "Cancer Statistics in Korea: Incidence, Mortality, Survival, and Prevalence in 2013" 대한암학회 48 (48): 436-450, 2016

40 조대현, "Bulnesia sarmienti 추출물의 항산화 및 항암효과" 한국식품위생안전성학회 22 (22): 120-126, 2007

41 Wu XD, "Bioactive sesquiterpenoids from the flowers of Inula japonica" 129 : 68-76, 2016

42 Li M, "Bigelovin triggered apoptosis in colorectal cancer in vitro and in vivo via upregulating death receptor 5 and reactive oxidative species" 7 : 42176-, 2017

43 Rajavel T, "Beta sitosterol and daucosterol(phytosterols identified in Grewia tiliaefolia) perturbs cell cycle and induces apoptotic cell death in A549 cells" 7 : 3418-, 2017

44 Jin-Hyuk Choi, "Bax Protein in Cancer Treatment" Korean Medical Association (KAMJE) 50 (50): 1016-, 2007

45 김지혜, "Apoptotic Effect of Rubia cordifolia Dichloromethane Extracts on Human Acute Jurkat T Cells" 한국생명과학회 19 (19): 163-168, 2009

46 Nagate S, "Apoptotic DNA fragmentation" 256 : 12-18, 2000

47 Ham SS, "Antimutagenicity and cytotoxicity of Artemisia iwayomogi kitamura extracts" 27 : 157-162, 1998

48 Hong JY, "Anticancer properties of Astilbe rubra extracts on AsPC-1 human pancreatic cancer cells" 32 : 21-28, 2015