The EST Study of the Peri-implanting Porcine Embryos

국문 초록 (Abstract)

임신초기의 배아는 peri-implantation 단계에서 매우 극적인 형태학적변화가 일어나는데, 이는 임신 인식 인자로 작용하는 배아에서 제공된 signal(s)에 의해서 시작되어지며, 나아가서 모성자궁과 배아의 상호 신호전달이 임신의 시작과 유지에 필수적인 인자로 작용한다. 배아형태의 급격한 리모델링에 관련된 세포학적, 생화학적, 유전학적 연구를 위하여, 또한 자궁과 배아의 상호 신호전달에 관여하는 잠재적 유전자 군을 발굴하기 위하여, peri-implantation 시기의 돼지배아를 이용하여 expresses sequences tag (EST) 분석을 실행하였다. 돼지배아 EST 분석으로 임신초기 특히 전 착상 단계에서 발현되는 유전자들의 카탈로그(Transcriptome)를 작성하였다. 그중에서 6개의 clone을 선택하여 그 발현 양식을 배아 및 자궁 등에서 관찰한 결과, 각각의 유전자들은 조직, 세포 유형 및 임신시기에 따른 특이적인 발현 현상을 나타내었다. 본 연구결과는, 배아와 자궁 내막에서의 유전자 발현이 임신 시기에 따라서 다이내믹한 상호 조절 작용을 하고 있음을 나타낸다. 이는 전 착상단계의 모성자궁에서 배아와 자궁 내막의 상호 신호전달이 전 착상 단계의 배아의 급격한 형태학적 변화를 가능하게하고 또한 착상에 필요한 적절한 자궁내부 환경을 제공하고 있음을 보여준다.

다국어 초록 (Multilingual Abstract)

A dramatic morphological change of embryos occurs at peri-implantation. Maternal and embryonic cross-talk during this period, initiated by signals from embryo(s), provides signals for maternal recognition of pregnancy and establishing and maintaining the pregnancy. However, the cellular, biochemical and genetic processes that direct embryo remodeling in mammalian species are not well studied or understood. In order to identify potential genes responsible for morphological change and cross-talk between embryo and uterus, an initial EST analysis was performed. A catalog of expressed genes (Transcriptome) from the d12 peri-implanting porcine embryos was constructed. Six clones were chosen from the initial ESTs for elucidation of their expression patterns during embryogenesis in early pregnancy. A number of these genes demonstrated unique expression profiles in a tissue, cell-type, and temporal fashion, indicating dynamic regulation of embryonic and endometrial gene expressions at different stages of pregnancy. Cross-talk between the embryo and endometrium of the pregnant uterus has provided a suitable micro-environment for the embryo’s rapid and dramatic morphological changing process at the peri-implantation stage.

목차 (Table of Contents)

• Introduction
• Materials and Methods
• Results and Discussion
• References
• 초록

참고문헌 (Reference)

1 Chwetzoff, S, "Ubiquitin is physiologically induced by interferons in luminal epithelium of porcine uterine endometrium in early pregnancy: global RT-PCR cDNA in place of RNA for differential display screening" 405 : 148-152, 1997

2 Barnard, G. F, "Ubiquitin fusion proteins are overexpressed in colon cancer but not in gastric cancer" 1272 : 147-153, 1995

3 Strauss, J. F. 3rd, "The steroidogenic acute regulatory protein (StAR): a window into the complexities of intracellular cholesterol trafficking" 54 : 369-394, 1999

4 Fujita, T, "Senescence marker protein-30 (SMP30): structure and biological function" 254 : 1-4, 1999

5 Inada, H., "QM is a novel zinc binding transcription regulatory protein: its binding to c-Jun is regulated by zinc ions and phosphorylation by protein kinase C" 230 : 331-334, 1997

6 Lee, C. Y, "Proteolysis of insulin-like growth factor-binding proteins (IGFBPs) within the pig uterine lumen associated with peri-implantation conceptus development" 112 : 369-377, 1998

7 Pilon, N, "Porcine and bovine steroidogenic acute regulatory protein (StAR) expression during gestation" 138 : 1085-1091, 1997

8 Oshima, R. G, "Oncogenic regulation and function of keratins 8 and 18" 15 : 445-471, 1996

9 Nilsson, J. A, "Myc pathways provoking cell suicide and cancer" 22 : 9007-9021., 2003

10 Graddy, L. G, "Multiple isoforms of porcine aromatase are encoded by three distinct genes" 73 : 49-57, 2000

11 Miller, W. L, "Molecular pathology and mechanism of action of the steroidogenic acute regulatory protein" 69 : 131-141, 1999

12 Choi, I, "Molecular cloning of cytochrome P450 aromatase complementary deoxyribonucleic acid from periimplantation porcine and equine blastocysts identifies multiple novel 5'-untranslated exons expressed in embryos, endometrium, and placenta" 137 : 1457-1467, 1996

13 Baribault, H, "Mid-gestational lethality in mice lacking keratin 8" 7 : 1191-1202, 1993

14 Pollack, S. E, "Localization of the steroidogenic acute regulatory protein in human tissues" 82 : 4243-4251, 1997

15 Bircan, S, "Immunohistochemical analysis of c-myc, c-jun and estrogen receptor in normal, hyperplastic and neoplastic endometrium" 11 : 32-39, 2005

16 Geisert, R. D, "Expression of messenger RNAs encoding insulin-like growth factor-I, -II, and insulin-like growth factor binding protein-2 in bovine endometrium during the estrous cycle and early pregnancy" 45 : 975-983, 1991

17 Fujita, T, "Expression and structure of senescence marker protein-30 (SMP30) and its biological significance" 107 : 271-280, 1999

18 Dubois, D. H, "Effect of porcine conceptus secretory proteins on in vitro secretion of prostaglandins-F2 alpha and E2 from luminal and myometrial surfaces of endometrium from cyclic and pseudopregnant gilts" 41 : 283-301, 1991

19 Nguyen, Y. H, "Assembly of the QM protein onto the 60S ribosomal subunit occurs in the cytoplasm" 68 : 281--285, 1998

20 Mills, A. A, "Analysis of the pattern of QM expression during mouse development" 64 : 161-171, 1999