Fabrication and Characterization of Electrospun Scaffold Based on Polycaprolactone-Aloe vera and Polyvinyl Alcohol for Skin Tissue Engineering

다국어 초록 (Multilingual Abstract)

In the present work, the biodegradable nanofibrous scaffolds containing poly(caprolactone) (PCL), poly(vinylalcohol) (PVA) and aloe vera (AV), through two nozzles electrospinning method, were prepared and characterized byscanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement and mechanical analysis.
The biocompatibility and cell growth were evaluated using MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide) assay. Adding poly(vinyl alcohol) (PVA) and poly(caprolactone) (PCL) boosted the electrospinability of aloe vera(AV) solution and mechanical features of the scaffolds. According to the Scanning electron microscopy (SEM) results,uniform PVA/AV-PCL nanofibers were fabricated with the average diameter about 119±11.78 nm. Fourier-transform infraredspectroscopy) FTIR (confirmed the presence of functional groups of scaffolds. The results of the contact angle showed thatby adding aloe vera, the hydrophilicity increased. The results showed that the tensile strength of samples with 6 mg/ml AV,reached to 2.02 MPa. MTT results showed that all samples had a cell viability of over 80 %. It can be concluded thatnanofibrous scaffolds with concentration of 6 mg/ml, distance between syringe tip/collector of 15 cm, feed rate of 3 ml/h andapplied voltage of 12 kV, have appropriate properties for skin tissue engineering by stimulating the fibroblast cells andtherefore accelerating the regeneration processes of damaged skin.

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