<P><B>Abstract</B></P> <P>Biodiesel production using oleaginous yeasts, such as <I>Yarrowia lipolytica</I>, has recently attracted strong attention in terms of lipid yield and productivity. However, efficient...
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https://www.riss.kr/link?id=A107443043
2019
-
SCIE,SCOPUS
학술저널
61-67(7쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P><B>Abstract</B></P> <P>Biodiesel production using oleaginous yeasts, such as <I>Yarrowia lipolytica</I>, has recently attracted strong attention in terms of lipid yield and productivity. However, efficient...
<P><B>Abstract</B></P> <P>Biodiesel production using oleaginous yeasts, such as <I>Yarrowia lipolytica</I>, has recently attracted strong attention in terms of lipid yield and productivity. However, efficient methods of lipid extraction from oleaginous yeasts are required to further improve the economic feasibility of yeast-based biodiesel production. We investigated the effectiveness of selected switchable hydrophilicity solvents (SHSs), namely, N-ethylbutylamine (EB), N-dipropylamine (DP), and N,N-dimethylcyclohexyl-amine (DMCHA), for lipid extraction from the oleaginous yeast <I>Y. lipolytica</I>. Regardless of using harvested cells or culture broth, DMCHA and EB effectively extracted lipids from <I>Y. lipolytica</I>, resulting in up to 13% higher lipid yields than that obtained by conventional organic solvent extraction using a chloroform/methanol mixture. The lipid extracts obtained by the SHSs showed fatty acid compositions comparable to that obtained with the conventional solvent, thus assuring the quality of biodiesel produced through this method. After switching processes by purging with CO<SUB>2</SUB> and N<SUB>2</SUB>, the lipids and solvents were successfully recovered, demonstrating the practicability of lipid extraction using SHSs. Consequently, this study provides an effective lipid extraction method for <I>Y. lipolytica</I> that could significantly improve energy efficiency and economic feasibility of an oleaginous-yeast-based biodiesel production process.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lipids were extracted from <I>Yarrowia lipolytica</I> by SHSs (DMCHA, EB, and DP). </LI> <LI> DMCHA and EB showed up to 13% higher yields relative to the conventional method. </LI> <LI> Lipids were effectively extracted from both harvested wet cells and culture broth. </LI> <LI> This extraction method would improve energy efficiency and economic feasibility. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>