Biological controli of root-knot nematodes using Paecilomyces Lilacinus = Biological controli of root-knot nematodes using Paecilomyces Lilacinus

다국어 초록 (Multilingual Abstract)

A nematode-pathogenic fungi, Paecilomyces lilacinus, was tested for selecting a promising biological control technique against root knot nematodes, Meloidogyne spp. We screened eleven strains of P. liacinus originated form Peru, India, and Korea by a petri-dish method. A petri-dish bioassay was constructed by consisting of sterile soil (40g), nematode eggs, testing solution (1me), and sterile distilled water (3me) in a 9cm plastic dish. THe dish were transferred to 25℃ incubator for 10 days, and then recorded by counting the number of hatched larvae. THe screening bioassays were conducted on three different species of M. hapla, originated from Peru, India, and Korea by a petri-dish method. A petri-dish bioassay was constructed by consisting of sterile soil (40g), nematode eggs, testing solution (1me), and sterile distilled water (3me) in a 9cm plastic dish. THe dish were transferred to 25℃ incubator for 10 days, and then recorded by counting the number of hatched larvae. The screening bioassays were conducted on three different species of M. hapla, M. arenaria, and M. incognita. Four strains of P. Lilacinus (P1, P3, P4, and Pv) were effective to inhibit the nematode egg development. To determine the effective spray concentration of these fungi, 10me of three different concentrations (10(6) 10(7), and 10(8) spores/me) were made and applied on each tomato pot. The most effective spray concentration was 10(7) spores/me, at which more than 80% of control efficacies were recorded. We applied the selected parasitic fungi to cucumber field at 20me of a concentration of 10(7) spores per 1m2. Different selected strains of P. lilacinus gave significant control efficacies, but varied from 71% to 78%. These resuits indicate that our selected parasitic fungi, P. Lilacinus, gave significant control efficacy, so we applied the selected P-1 and P-v parasitic fungi to oriental melon field at 2e of a concentration of 10(5), 10(6) and 10(7) spores per 3m2. These results indicate that our selected parasitic fungi, P-1 and P-v gave significant control efficacies, but need to be further developed to give a stable control effect.