Germination response to the treatment of temperature, low temperature period, red light and various plant growth regulators are studied by the use of the seeds of Daphniphyllum macropodum. Also physiology in seed germination was studies in relation to...
Germination response to the treatment of temperature, low temperature period, red light and various plant growth regulators are studied by the use of the seeds of Daphniphyllum macropodum. Also physiology in seed germination was studies in relation to characterization of the water uptake and changes of seed-stored substances to the treatment of low temperature and GA during seed germination.
The experimental results from the studies are briefed as the following compendia.
1. Effects of temperature, low temperature period, red light and various plant growth regulators on the seed germination.
1) Although the seed germination did not take place in 15 weeks when done at 25℃, germination of Daphniphyllum macropodum seeds are promoted at 15℃, and inhibition was greater as temperature increased.
2) Promotion of seed germination was greater as low temperature period prolonged, and the treatment of 4 weeks low temperature was not more significantly influence on its promotion than the control.
3) Red light promoted the seed germination at 15℃, but lost its promotive effect at over 20℃. And there was a greater promotion in dark than the light.
4) The GA treatment at 10mg/l greatly promoted seed germination, and inhibition was greater as concentration increased.
5) The NAA treatment under 10mg/l was not more significantly effect than the control, and germination percent decreased greater than the control as concentration increased.
6) The ethephon treatment at 1mg/l greatly promoted seed germination, but within the range of 25 to 100mg/l, was almost as germination percent as the control.
7) The BA treatment at 1mg/l greatly promoted seed germination, and also, the other concentration was more effective than the control.
2. Effects of GA and low temperature treatment on water uptake and change of seed-stored substances during seed germination.
1) Low temperature or GA treatment promoted greater seed germination than the control. However combined treatment with both of them demonstrated greater level of germination than either low treatment or GA alone.
2) The control, low temperature, GA and low temperature plus GA treatment showed three stages of water uptake during seed germination, namely 1) rapid increase, 2) steady, and 3) increase in the water uptake. There was a gradual increase in the third increase of the control than the other treatment.
3) The content of protein and crude fat decreased more rapidly in low temperature plus GA treatment than the control. Although its content was more decreased in low temperature or GA alone than the control, there was more decrease in the combined treatment than either low temperature of GA alone.
4) After one week of imbibition, the content of carbohydrat and total sugar was decreased more rapidly in low temperature, GA, and low temperature plus GA treatment than the control.
5) During seed germination, the content of starch and sucrose was decreased in the all treatments, but due to the much consumption of energy, its content was more decreased in low temperature plus GA than either low temperature or GA alone.