Recent Advances in Microfluidic-Based Microphysiological Systems

다국어 초록 (Multilingual Abstract)

Microphysiological systems are in vitro tissue and organ models that present unique opportunities across different disciplines, such as biology, medicine, and engineering. These systems are preferred over two-dimensional simplistic cell cultures and animal models with poor translatability. Microphysiological systems can facilitate the understanding of human physiology and pathophysiology by recapitulating specific organ structures and functions. Recent advances in microphysiological systems employ microfluidic technologies to gain deeper insights into more complex in vivo physiological phenomena and mechanisms. More specifically, microfluidic technologies allow the development of more realistic physiological conditions.
This review highlights recent advances in microfluidic-based microphysiological systems and comprehensively discusses the relationship between the design and operation of microfluidic systems for mimicking physiological microenvironments.
Moreover, several representative examples and their potential biological applications are described and explained in detail.
Finally, the review concludes with current perspectives, limitations, and prospects for further improving the microfluidicbased microphysiological systems.

참고문헌 (Reference)

1 Xie, R, "h-FIBER: Microfluidic topographical hollow fiber for studies of glomerular filtration barrier" 6 : 903-912, 2020

2 Harris, T.W, "WormBase: a multi-species resource for nematode biology and genomics" 32 : D411-417, 2004

3 Wang, Y.I, "UniChip enables long-term recirculating unidirectional perfusion with gravity-driven flow for microphysiological systems" 18 : 2563-2574, 2018

4 Discher, D.E, "Tissue cells feel and respond to the stiffness of their substrate" 310 : 1139-1143, 2005

5 Shamir, E.R, "Three-dimensional organotypic culture: experimental models of mammalian biology and disease" 15 : 647-664, 2014

6 Fang, Y, "Three-dimensional cell cultures in drug discovery and development" 22 : 456-472, 2017

7 Nüsslein-Volhard, C, "The zebrafish issue of development" 139 : 4099-4103, 2012

8 Baumeister, R, "The worm in us – Caenorhabditis elegans as a model of human disease" 20 : 147-148, 2002

9 Wells, R.G, "The role of matrix stiffness in regulating cell behavior" 47 : 1394-1400, 2008

10 Wirtz, D, "The physics of cancer: the role of physical interactions and mechanical forces in metastasis" 11 : 512-522, 2011

11 Brenner, S, "The genetics of Caenorhabditis elegans" 77 : 71-94, 1974

12 Sulston, J.E, "The embryonic cell lineage of the nematode Caenorhabditis elegans" 100 : 64-119, 1983

13 Song, Y, "The effect of different surface treatment methods on the physical, chemical and biological performances of a PGA scaffold" 9 : 20174-20184, 2019

14 Sin, A, "The design and fabrication of three-chamber microscale cell culture analog devices with integrated dissolved oxygen sensors" 20 : 338-345, 2004

15 Aisenbrey, E.A, "Synthetic alternatives to Matrigel" 5 : 539-551, 2020

16 Liu, M, "Stimulation of fetal rat lung cell proliferation in vitro by mechanical stretch" 263 : L376-L383, 1992

17 Zhang, R, "Stereolithographic hydrogel printing of 3D culture chips with biofunctionalized complex 3D perfusion networks" 17 : 4273-4282, 2017

18 Schittny, J.C, "Spontaneous peristaltic airway contractions propel lung liquid through the bronchial tree of intact and fetal lung explants" 23 : 11-18, 2000

19 Rolland, J.P, "Solvent-resistant photocurable liquid fluoropolymers for microfluidic device fabricatio" 126 : 2322-2323, 2004

20 Levental, I, "Soft biological materials and their impact on cell function" 3 : 299-306, 2007

21 Benam, K.H, "Small airwayon- a-chip enables analysis of human lung inflammation and drug responses in vitro" 13 : 151-157, 2016

22 Huh, D, "Reconstituting organ-level lung functions on a chip" 328 : 1662-1668, 2010

23 Frey, O, "Reconfigurable microfluidic hanging drop network for multitissue interaction and analysis" 5 : 4250-, 2014

24 Nyholm, S.V, "Recognition between symbiotic Vibrio fischeri and the haemocytes of Euprymna scolopes" 11 : 483-493, 2009

25 Yang, K, "Recapitulation of in vivo-like paracrine signals of human mesenchymal stem cells for functional neuronal differentiation of human neural stem cells in a 3D microfluidic system" 63 : 177-188, 2015

26 Dincau, B, "Pulsatile flow in microfluidic systems" 16 : 1904032-, 2020

27 Rajan, S.A.P, "Probing prodrug metabolism and reciprocal toxicity with an integrated and humanized multi-tissue organ-on-a-chip platform" 106 : 124-135, 2020

28 McLean, I.C, "Powering ex vivo tissue models in microfluidic systems" 18 : 1399-1410, 2018

29 Lasprilla, A.J, "Poly-lactic acid synthesis for application in biomedical devices—A review" 30 : 321-328, 2012

30 Deiss, F, "Platform for high-throughput testing of the effect of soluble compounds on 3D cell cultures" 85 : 8085-8094, 2013

31 Vitale, A, "Photopolymerization of a perfluoropolyether oligomer and photolithographic processes for the fabrication of microfluidic devices" 48 : 1118-1126, 2012

32 Tejavibulya, N, "Personalized disease models on a chip" 3 : 416-418, 2016

33 Ng, K, "Paper-based cell culture platform and its emerging biomedical applications" 20 : 32-44, 2017

34 Toepke, M.W, "PDMS absorption of small molecules and consequences in microfluidic applications" 6 : 1484-1486, 2006

35 Henry, O.Y.F, "Organs-on-chips with integrated electrodes for trans-epithelial electrical resistance (TEER) measurements of human epithelial barrier function" 17 : 2264-2271, 2017

36 Ronaldson-Bouchard, K, "Organs-on-achip: A fast track for engineered human tissues in drug development" 22 : 310-324, 2018

37 Simian, M, "Organoids: a historical perspective of thinking in three dimensions" 216 : 31-40, 2017

38 Fatehullah, A, "Organoids as an in vitro model of human development and disease" 18 : 246-254, 2016

39 Skardal, A, "Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling" 21 : 1399-1411, 2016

40 Lancaster, M.A, "Organogenesisin a dish: Modeling development and disease using organoid technologies" 345 : 1247125-, 2014

41 Wu, Q, "Organ-on-a-chip: recent breakthroughs and future prospects" 19 : 9-, 2020

42 Ma, C, "Organ-on-a-chip: a new paradigm for drug development" 42 : 119-133, 2021

43 Zheng, F, "Organ-on-a-chip systems: microengineering to biomimic living systems" 12 : 2253-2282, 2016

44 Jang, M, "On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro" 9 : 034113-, 2015

45 Grigoryan, B, "Multivascular networks and functional intravascular topologies within biocompatible hydrogels" 364 : 458-464, 2019

46 Zhang, Y.S, "Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors" 114 : E2293-E2302, 2018

47 Sone, N, "Multicellular modeling of ciliopathy by combining iPS cells and microfluidic airway-on-a-chip technology" 13 : eabb1298-, 2021

48 Skarda, A, "Multi-tissue interactions in an integrated three-tissue organ-on-a-chip platform" 7 : 8837-, 2017

49 Oleaga, C, "Multi-organ toxicity demonstration in a functional human in vitro system composed of four organs" 6 : 20030-, 2016

50 Sticker, D, "Multi-layered, membrane-integrated microfluidics based on replica molding of a thiol–ene epoxy thermoset for organ-on-achip applications" 15 : 4542-4554, 2015

51 Vollertsen, A.R, "Modular operation of microfluidic chips for highly parallelized cell culture and liquid dosing via a fluidic circuit board" 6 : 107-, 2020

52 Cecchelli, R, "Modelling of the blood–brain barrier in drug discovery and development" 6 : 650-661, 2007

53 Sontheimer-Phelps, A, "Modelling cancer in microfluidic human organs-on-chips" 19 : 65-81, 2019

54 Unger, C, "Modeling human carcinomas: physiologically relevant 3D models to improve anti-cancer drug development" 79–80 : 50-67, 2014

55 Lee, S.H, "Microtechnology-based multi-organ models" 4 : 46-, 2017

56 Khetani, S.R, "Microscale culture of human liver cells for drug development" 26 : 120-126, 2008

57 Li, C.Y, "Micropatterned cell–cell interactions enable functional encapsulation of primary hepatocytes in hydrogel microtissues" 20 : 2200-2212, 2014

58 Bhatia, S.N, "Microfluidic organs-on-chips" 32 : 760-772, 2014

59 Moradi, E, "Microfluidic organ-on-a-chip models of human liver tissue" 116 : 67-83, 2020

60 Humayun, M, "Microfluidic lung airway-on-a-chip with arrayable suspended gels for studying epithelial and smooth muscle cell interactions" 18 : 1298-1309, 2018

61 Virumbrales-Muñoz, M, "Microfluidic lumen-based systems for advancing tubular organ modeling" 49 : 6402-6442, 2020

62 Park, J.W, "Microfluidic culture platform for neuroscience research" 1 : 2128-2136, 2006

63 Lam, M.T, "Microfeature guided skeletal muscle tissue engineering for highly organized 3-dimensional free-standing constructs" 30 : 1150-1155, 2009

64 Carmona-Fontaine, C, "Metabolic origins of spatial organization in the tumor microenvironment" 114 : 2934-2939, 2017

65 Prodanov, L, "Long-term maintenance of a microfluidic 3D human liver sinusoid" 113 : 241-246, 2016

66 Beckwitt, C.H, "Liver ‘organ on a chip" 363 : 15-25, 2018

67 Jellali, R, "Liver and kidney cells cultures in a new perfluoropolyether biochip" 229 : 396-407, 2016

68 Kratz, S.R.A, "Latest trends in biosensing for microphysiological organs-on-a-chip and body-on-a-chip systems" 9 : 110-, 2019

69 Haffter, P, "Large scale genetics in a small vertebrate, the zebrafish" 40 : 221-227, 1996

70 Shetty, S.D, "Investigation of mechanical properties and applications of polylactic acids—a review" 6 : 112002-, 2019

71 Gori, M, "Investigating nonalcoholic fatty liver disease in a liver-on-a-Chip microfluidic device" 11 : e0159729-, 2016

72 Edington, C.D, "Interconnected microphysiological systems for quantitative biology and pharmacology studies" 8 : 4530-, 2018

73 Gruber, P, "Integration and application of optical chemical sensors in microbioreactors" 17 : 2693-2712, 2017

74 Lind, J.U, "Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing" 16 : 303-308, 2017

75 Barboni, B, "Indirect co-culture with tendons or tenocytes can program amniotic epithelial cells towards stepwise tenogenic differentiation" 7 : e30974-, 2012

76 Sielken, R.L., Jr, "Incorporating additional biological phenomena into two-stage cancer models" 387 : 237-260, 1994

77 Yeon, J.H, "In vitro formation and characterization of a perfusable three-dimensional tubular capillary network in microfluidic devices" 12 : 2815-2822, 2012

78 Carraro, A, "In vitro analysis of a hepatic device with intrinsic microvascular-based channels" 10 : 795-805, 2008

79 Park, T.-E, "Hypoxiaenhanced Blood-Brain Barrier Chip recapitulates human barrier function and shuttling of drugs and antibodies" 10 : 2621-, 2019

80 안수환 ; 한승엽 ; 조승우, "Hydrogel-integrated Microfluidic Systems for Advanced Stem Cell Engineering" 한국바이오칩학회 13 (13): 306-322, 2019

81 Marx, U, "Human-on-a-chip’ developments: a translational cutting-edge alternative to systemic safety assessment and effi- ciency evaluation of substances in laboratory animals and man?" 40 : 235-257, 2012

82 Abaci, H.E, "Human-on-a-chip design strategies and principles for physiologically based pharmacokinetics/pharmacodynamics modeling" 7 : 383-391, 2015

83 Jang, K.-J, "Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment" 5 : 1119-1129, 2013

84 Kim, H.J, "Human guton- a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow" 12 : 2165-2174, 2012

85 Wang, Y, "Human brain organoidon- a-chip to model prenatal nicotine exposure" 18 : 851-860, 2018

86 Jeon, J.S, "Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation" 112 : 214-219, 2015

87 Kim, H.J, "Gut-on-a-Chip microenvironment induces human intestinal cells to undergo villus differentiation" 5 : 1130-1140, 2013

88 Hirama, H, "Glass-based organ-on-a-chip device for restricting small molecular absorption" 127 : 641-646, 2019

89 Seok, J, "Genomic responses in mouse models poorly mimic human inflammatory diseases" 110 : 3507-3512, 2013

90 Poulin, G, "Genome-wide RNAi screens in Caenorhabditis elegans: impact on cancer research" 23 : 8340-8345, 2004

91 Chen, Y.-A, "Generation of oxygen gradients in microfluidic devices for cell culture using spatially confined chemical reactions" 11 : 3626-3633, 2011

92 Young, E.W.K, "Fundamentals of microfluidic cell culture in controlled microenvironments" 39 : 1036-1048, 2010

93 Huh, D, "From 3D cell culture to organs-on-chips" 21 : 745-754, 2011

94 Homan, K.A, "Flow-enhanced vascularization and maturation of kidney organoids in vitro" 16 : 255-262, 2019

95 Daemi, H, "Facile fabrication of sulfated alginate electrospun nanofibers" 198 : 481-485, 2018

96 Steenbergen, R, "Establishing normal metabolism and differentiation in hepatocellular carcinoma cells by culturing in adult human serum" 8 : 11685-, 2018

97 Pagani, L.S, "Environmental tobacco smoke exposure and brain development: The case of attention deficit/hyperactivity disorder" 44 : 195-205, 2014

98 Kang, S.-M, "Engineered microsystems for spheroid and organoid studies" 10 : e2001284-, 2021

99 Kratochvil, M.J, "Engineered materials for organoid systems" 4 : 606-622, 2019

100 Shin, M, "Endothelialized networks with a vascular geometry in microfabricated poly(dimethyl siloxane)" 6 : 269-278, 2004

101 Sharma, K, "Dynamic persistence of UPEC intracellular bacterial communities in a human bladderchip model of urinary tract infection" 10 : e66481-, 2021

102 Hegde, M, "Dynamic interplay of flow and collagen stabilizes primary hepatocytes culture in a microfluidic platform" 14 : 2033-2039, 2014

103 Herland, A, "Distinct contributions of astrocytes and pericytes to neuroinflammation identified in a 3D human blood-brain barrier on a chip" 11 : e0150360-, 2016

104 Xu, J, "Differential regulation of extracellular matrix molecules by mechanical strain of fetal lung cells" 276 : L728-L735, 1999

105 Ricchi, M, "Differential effect of oleic and palmitic acid on lipid accumulation and apoptosis in cultured hepatocytes" 24 : 830-840, 2009

106 Jang, K, "Development of an osteoblast-based 3D continuous-perfusion microfluidic system for drug screening" 390 : 825-832, 2008

107 Baudoin, R, "Development of a renal microchip for in vitro distal tubule models" 23 : 1245-1253, 2007

108 Kasendra, M, "Development of a primary human small intestine-on-a-chip using biopsy-derived organoids" 8 : 2871-, 2018

109 Kim, H.J, "Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip" 113 : E7-E15, 2015

110 Chisolm, D.A, "Connections between metabolism and epigenetics in programming cellular differentiation" 36 : 221-246, 2018

111 Song, J.W, "Computer-controlled microcirculatory support system for endothelial cell culture and shearing" 77 : 3993-3999, 2005

112 Gelse, K, "Collagens—structure, function, and biosynthesis" 55 : 1531-1546, 2003

113 Chevallay, B, "Collagen-based biomaterials as 3D scaffold for cell cultures: applications for tissue engineering and gene therapy" 38 : 211-218, 2000

114 Kim, S.A, "Co-culture of 3D tumor spheroids with fibroblasts as a model for epithelial–mesenchymal transition in vitro" 335 : 187-196, 2015

115 Booth, R, "Characterization of a microfluidic in vitro model of the blood-brain barrier (μBBB)" 12 : 1784-1792, 2012

116 Mahler, G.J, "Characterization of a gastrointestinal tract microscale cell culture analog used to predict drug toxicity" 104 : 193-205, 2009

117 Bhatia, S.N, "Cell and tissue engineering for liver disease" 6 : 24-52, 2014

118 Wirtz, H.R.W, "Calcium mobilization and exocytosis after one mechanical stretch of lung epithelial cells" 250 : 1266-1269, 1990

119 Kim, Y, "Caenorhabditis elegans immune conditioning with the probiotic bacterium Lactobacillus acidophilus strain NCFM enhances gram-positive immune responses" 80 : 2500-2508, 2012

120 Esch, M.B, "Body-on-achip simulation with gastrointestinal tract and liver tissues suggests that ingested nanoparticles have the potential to cause liver injury" 14 : 3081-3092, 2014

121 Ghaemmaghami, A.M, "Biomimetic tissues on a chip for drug discovery" 17 : 173-181, 2012

122 Radisic, M, "Beyond PDMS and membranes: new materials for organ-on-a-chip devices" 7 : 2861-2863, 2021

123 Shanks, N, "Are animal models predictive for humans?" 4 : 1-20, 2009

124 Hammerschmidt, S, "Apoptosis and necrosis induced by cyclic mechanical stretching in alveolar type II cells" 30 : 396-402, 2004

125 Scaffaro, R, "Antimicrobial additives for poly (lactic acid) materials and their applications: current state and perspectives" 102 : 7739-7756, 2018

126 Stirman, J.N, "Animal microsurgery using microfluidics" 25 : 24-29, 2014

127 Lee, K.Y, "Alginate: properties and biomedical applications" 37 : 106-126, 2012

128 Kang, S.M, "Alginate microencapsulation for three-dimensional in vitro cell culture" 7 : 2864-2879, 2021

129 Chang, J.-E, "Air-interface condition promotes the formation of tight corneal epithelial cell layers for drug transport studies" 17 : 670-676, 2000

130 Zhang, B, "Advances in organ-on-a-chip engineering" 3 : 257-278, 2018

131 Huh, D, "Acoustically detectable cellular-level lung injury induced by fluid mechanical stresses in microfluidic airway systems" 104 : 18886-18891, 2007

132 Waters, C.M, "A system to impose prescribed homogenous strains on cultured cells" 91 : 1600-1610, 1985

133 Ahmed, S, "A review on chitosan centred scaffolds and their applications in tissue engineering" 116 : 849-862, 2018

134 Ali, A, "A review on chitosan and its nanocomposites in drug delivery" 109 : 273-286, 2018

135 Li, R, "A rapidly fabricated microfluidic chip for cell culture" 54 : 523-530, 2016

136 Satoh, T, "A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation platform" 18 : 115-125, 2017

137 Jang, K.-J, "A multi-layer microfluidic device for efficient culture and analysis of renal tubular cells" 10 : 36-42, 2010

138 Shah, P, "A microfluidics-based in vitro model of the gastrointestinal human–microbe interface" 7 : 11535-, 2016

139 Rennert, K, "A microfluidically perfused three dimensional human liver model" 71 : 119-131, 2015

140 Midwoud, P.M, "A microfluidic approach for in vitro assessment of interorgan interactions in drug metabolism using intestinal and liver slices" 10 : 2778-2786, 2010

141 Sung, J.H, "A micro cell culture analog (μCCA) with 3-D hydrogel culture of multiple cell lines to assess metabolism- dependent cytotoxicity of anti-cancer drugs" 9 : 1385-1394, 2009

142 Stucki, A.O, "A lung-on-a-chip array with an integrated bio-inspired respiration mechanism" 15 : 1302-1310, 2015

143 Schulte, V.A, "A hydrophobic perfluoropolyether elastomer as a patternable biomaterial for cell culture and tissue engineering" 31 : 8583-8595, 2010

144 Florian, S, "A human organoid system that self-organizes to recapitulate growth and differentiation of a benign mammary tumor" 116 : 11444-11453, 2019

145 Huh, D, "A human disease model of drug toxicity-induced pulmonary edema in a lung-on-a-chip microdevice" 4 : 159ra147-, 2012

146 Wang, L, "A disease model of diabetic nephropathy in a glomerulus-on-a-chip microdevice" 17 : 1749-1760, 2017

147 Jalili-Firoozinezhad, S, "A complex human gut microbiome cultured in an anaerobic intestine-on-achip" 3 : 520-531, 2019

148 Rolig, A.S, "A bacterial immunomodulatory protein with lipocalin-like domains facilitates host–bacteria mutualism in larval zebrafish" 7 : e37172-, 2018

149 Adriani, G, "A 3D neurovascular microfluidic model consisting of neurons, astrocytes and cerebral endothelial cells as a blood–brain barrier" 17 : 448-459, 2017

150 Schulze, T, "A 3D microfluidic perfusion system made from glass for multiparametric analysis of stimulus-secretioncoupling in pancreatic islets" 19 : 47-, 2017

151 Park, J, "3D miniaturization of human organs for drug discovery" 7 : 1700551-, 2018

152 김명지 ; 황동규 ; 장진아, "3D Pancreatic Tissue Modeling in vitro: Advances and Prospects" 한국바이오칩학회 14 (14): 84-99, 2020