난소 상피종양의 p53 단백 표현에 관한 면역조직화학적 연구 = Immunohistochemical Study of Expression of p53 Protein in Epithelial Ovarian Tumors

다국어 초록 (Multilingual Abstract)

p53, a nuclear protein, is believed to play an important role, through mutation and overexpression, in the progression of human malignant tumors. Mouse monoclonal antibody NCL-p53-DO7(Novocastra Laboratories Ltd., Newcastle, England) which specifically immunoprecipitates mutant-form p53 protein, was used to examine 38 parafifin-embedded ovarian tumor (five mucinous cystadenoma, one borderline serous cystadenoma, and seven serous cystadenocaricinoma) to find intranuclear expression of mutant-form p53 protein.
The results obtained are as follows:
1. In mucinous, and serous cystadenoma, expression of mutant-form p53 protein were negative.
2. In borderline mucinous cystadenoma, two out of eight of tumors(25%) showed expression of mutant-form p53 protein. However, borderline serous cystadenoma showed negative result.
3. In mucinous cystadenocarcinoma, three out of 11 tumors(27.3%) showed expression of mutant-form p53 protein, and three out of serous cystadenocarcinomas(42.9%) showed expression of p53 protein. And so, overexpression of p53 protein in serous cystadenocarcinoma was more frequent than mucinous cystadenocarcinoma.
4. In comparison between borderline and malignant tumors, approximately same percent of mucinous tumor showed overexpression of p53 protein. However, in serous tumors, borderline tumor that was studied was only one case, same comparison between tumors was impossible.
In this sutudy, overexpression of the p53 protein was observed in malignat epithelial tumors of ovary. And this observation suggests that the p53 tumor suppressor gene is involved in the tumorigenessis of malignat ovarian epithelial tumors.