Chitinase is a basic protein that catalyses the hydrolysis of the β-1,4 linkage of the N-acetyl-D-glucosamine polymer chitin, cDNA library construction carried out essentially by the dimer primer cDNA cloning method. Primary screening identified a fe...
Chitinase is a basic protein that catalyses the hydrolysis of the β-1,4 linkage of the N-acetyl-D-glucosamine polymer chitin, cDNA library construction carried out essentially by the dimer primer cDNA cloning method. Primary screening identified a few positive signals containing chitinase cDNA inserts. For strong expression of chitinase cDNA in tobacco or other plants, we were attached the CaMV 35S promoter to 5' terminal and NOS poly (A)^+ signal to 3' terminal of tobacco chitinase cDNA. This recombinent plasmid pPB 3 was transformed into A. tumefaciens LBA 4404 by triparental mating method.