Tooth transplantation has been the subject of extensive studies for many years. The literature is replete with the articles which describe auto-, homo-, and hetero-transplantation experiments in animals. Study in this field has progressed rapidly in r...
Tooth transplantation has been the subject of extensive studies for many years. The literature is replete with the articles which describe auto-, homo-, and hetero-transplantation experiments in animals. Study in this field has progressed rapidly in recent years, and some of the successful case reports of this transplantation experiments on human den-"-:ion tend to support: the assumption that this new philosophy will find a very valuable clinical applicability in the near future.
This report by the author concerns with this tooth germ transplantation on rabbits. With the hope of maintaining an ideal environment in this experiment, autotransplantation was employed, and bone marrow of tibia was selelcted as a site for this transplantation of tooth germ from the rabbit mandible.
Materials and method:
4 to 6 months old rabbits having body weight of 1, 800-2, 000 Gm. were selected first, and they were reselected after well-nourished of one month. 25% Urethane was injected in the vein of ear lobe in order to induce general anesthesia (2cc/500Gm.) and operation sites of mandible and tibia were additionally anesthetized by Xylocaine Hydrochloride with 1:50,000 Epinephrine in an attempt to have vasoconstriction effects.
According to usual manner, operation site was sterilized and incision was made along the inferior border of the mandible, exposing the bony surface exterior to the apex of tooth in the area where tooth germ is generally located. Tooth germ was removed carefully after bony window was excised, and the tooth germ was placed in the sterilized normal saline solution before the operation of transplantation site in tibia is complete. In the usual manner. tibia was prepared to receive this tooth germ from the mandible by creating a bony cavity which is slightly larger than the transplant itself (4X4mm) by cutting away bony wall of tibia into the marrow by using ##700 fissure bur so that this window can be exactly replaced back subsequent to the operation. Tibia having been ready, transplant was carefully placed in the transplantation site and bony window was placed back and operation was finished after suturing soft tissues.
Result
Observation was made of the histological changes that. took place in the apical region of the removed tooth germ and the fate of transplant in the tibia.
1. Osteoid tissue appeared in the apical region from which transplant was, removed 10 days after the operation. 20 days after the operation, regeneration of enamel organ as can be seen ,,in the -normal_ germ took place and appearance of Hertwig's epithelial sheath was noted.
2. In the earlier phase following the operation, severe hemorrhage and infiltration of round cells in the surroundings of transplants were the characteristics of changes. However, in some cases, there were observed the formations of enamel organ and normal dentin from the transplanted tooth germ. In the majority of cases, however, newly formed osteoid-dentin of irregular forms showed evidence of gradual resorption by the giant-cells that seemed to have appeared in the area after transplants where place in the tibia.