葛根黃蓮黃芩湯과 疎風活血湯이 HUVEC 내에 MCP-1, ICAM-1, VCAM-1 and eNOS의 유전자 발현량에 대해 미치는 영향 = The Effect of Galkunhwanglyeonhwanggum-tang (GGT) and Sopunghwalhyeol-tang (SPT) on Gene Expression Levels of MCP-1, ICAM-1, VCAM-1, and eNOS in HUVECs

다국어 초록 (Multilingual Abstract)

Objectives: The aim of this study was to compare the effects of Galkunhwanglyeonhwanggum-tang (GGT), and Sopunghwalhyeol-tang (SPT) on gene expression of MCP-1, ICAM-1, VCAM-1, and eNOS in human umbilical vein endothelial cells (HUVECs).
Methods: HUVECs were treated with GGT and SPT at concentrations of 50, 100, and 200 μg/mL. Gene expression of MCP-1, ICAM-1 ,VCAM-1, and eNOS in HUVECs was analyzed by the polymerase chain reaction (PCR), and electrophoresis was performed to verify the gene expression level.
Results: 1. MCP-1 gene expression was more strongly decreased by SPT than by GGT.
2. ICAM-1 and VCAM-1 gene expressions were more strongly decreased by SPT than by GGT 3. GGT significantly increased eNOS gene expression, but SPT did not.
Conclusions: These findings suggest that GGT and SPT regulate gene expression related to anti-inflammatory effects in HUVECs. Clinical application of these Korean medicines to diseases related to dyslipidemia, such as cardiovascular disease, will require additional in vivo experiments to verify the anti-inflammatory effects of GGT and SPT.

참고문헌 (Reference)

1 윤현정, "마우스 대식세포 및 사람 혈관 내피세포에서 오수유(Evodia officinalis DODE) 메탄올 추출물의 항염증 효과" 대한본초학회 23 (23): 29-38, 2008

2 전명숙, "마우스 Raw 264.7 세포에 대한 4종 대황류 추출물의 항염증 활성 비교" 한국약용작물학회 17 (17): 109-114, 2009

3 박승현, "다중절편 방사선단층촬영 결과에 기반한 한국 중년 남성에서의 관상동맥 석회화와 심혈관질환 위험인자와의 연관성" 한국농촌의학.지역보건학회 39 (39): 25-36, 2014

4 Bang HJ, "고지혈증의 처방에 관한 문헌적 고찰" 21 (21): 70-81, 1996

5 Jeong WY, "韓方臨床病理學" Yunglimsa 545-, 1998

6 黃度淵, "證脈方藥合編" Namsandang 125-126, 1992

7 沈金資, "沈氏尊生書" 自由出版社 309-317, 1980

8 국가통계포털, "○DB :보건·사회 ·복지 >보건 >사망원인통계" KOSIS

9 Gedeit RG, "Tumor necrosis factor-induced E-selectin expression on vascular endothelial cells" 24 (24): 1543-1546, 1996

10 Li J, "The role of inflammation in coronary artery calcification" 6 (6): 263-270, 2007

11 Harrington JR, "The role of MCP-1 in atherosclerosis" 18 (18): 65-66, 2000

12 Fuster V, "The pathogenesis of coronary artery disease and the acute coronary syndromes(1)" 326 (326): 242-250, 1992

13 Power CA, "The chemokine system : novel broad-spectrum therapeutic targets" 1 (1): 417-424, 2001

14 Wong ND, "Risk factors for long-term coronary prognosis after inital myocardial infarction" 130 (130): 469-480, 1989

15 Kang DY, "Patholgy" Komunsa 349-353, 2004

16 Nelken NA, "Monocyte chemoattractant protein-1 in human athero matous plaques" 88 (88): 1121-1127, 1991

17 Gosling J, "MCP-1 deficiency reduces susceptibility to atheroslcerosis in mice that overexpress human apolipoprotein B" 103 (103): 773-778, 1999

18 Mc Kenney JM, "Lipid management : tools for gettingto the goal" 7 (7): S299-S306, 2001

19 Stein O, "Is there a genetic basis for resistance to atherosclerosis?" 160 (160): 1-10, 2002

20 Stenvinkel P, "Interactions between inflammation, oxidative stress and endothelial dysfunction in end-stage renal disease" 13 (13): 144-148, 2003

21 Ito T, "Inflammatory cytokines and cardiovascular disease" 2 (2): 257-265, 2003

22 Liby P, "Inflammation in atherosclerosis" 420 (420): 868-874, 2002

23 Lee JH, "Ilustration of the TCM Diagnosis" Publications EuiBang 79-, 2004

24 Rong Y, "Ginko biloba attenuates oxidative stress in macrophages and endothelial cells" 20 (20): 121-127, 1996

25 Seino Y, "Expression of monocyte chemoattactant protien-1 in vascularb tissue" 7 (7): 575-579, 1995

26 Wen MC, "Efficacy and tolerability ofanti-asthma herbalmedicine intervention in adult patients with moderate-severe allergic asthma" 116 (116): 517-524, 2005

27 Heo J, "Dongeuibogam" Namsandang 86-90, 1989

28 Takeya M, "Detection of monocyte chemoattractant protein-1 in human atherosclerotic lesions by an anti-monocyte chemoattractant protein-1 monoclonal antibody" 24 (24): 534-539, 1993

29 The Warfarin-Aspirin Symptomatic Intracranial Disease(WASID) Trial Investigatiors, "Design, progress and challenges of double-blind trial of warfarin versus aspirin for symptomatic intracranial arterial stenosis" 22 (22): 106-117, 2003

30 Miettinen TA, "Cholesterol absorption inhibition : astrategy for cholesterol-lowering therapy" 55 (55): 710-716, 2001

31 Baggiolini M, "Chemokines in inflammation and immunity" 21 (21): 418-420, 2000

32 Baran D, "Cell adhesion molecule expression in murine lupus-like nephritis induced by lipopolysaccharide" 84 : 167-176, 2000

33 Kim WS, "Berberine improves lipid dysregulation in obesity by controlling central and peripheral AMPK activity" 296 (296): 812-819, 2009

34 Imhof BA, "Basic mechanism of leukocyte migration" 29 (29): 614-621, 1997