Gold-some Amplified Plasmonic Immunoassay based on Viral Fusion for Point-of-Care Testing of Respiratory Virus

다국어 초록 (Multilingual Abstract)

We propose a plasmonic immunoassay based on a target specific organic-inorganic hybrid nanoparticle with an antibody-functionalized glass substrate. The hybrid nanoparticle (Gold-somes) encapsulates gold nanoparticles (GNPs) which are used to amplify the detection signal. In the presence of the virus, the glass substrate will capture the target, and viral fusion of Gold-somes will follow. During the viral fusion, encapsulated GNPs will escape the probe. With the addition of reducing agents, freed GNPs will form aggregates, resulting in a color change with respect to a peak shift. We conducted experiments to synthesize the hybrid nanoparticles (Gold-somes) and to optimize the reaction environment for the glass substrate. Eventually, we expect our plasmonic immunoassay platform be applied for point-of-care testing via colorimetric virion detection.