In Vitro Inhibitory Effect of Protopanaxadiol Ginsenosides on Tumor Necrosis Factor(TNF) : α생성 억제효과 및 조절 연구 αProduction and its Modulation by known TNF-αAntagonists = Protopanaxadiol Ginsenosides의 in vitro Tumor Necrosis Factor(TNF)

다국어 초록 (Multilingual Abstract)

Ginsenosides are the major principles of Panax ginseng C. A. Meyer(Araliaceae) used as a mild oriental folk medicine. In this report, we have examined the inhibitory potency of protopanaxadiol ginsenosides (PPDGS) such as Rb1, Rb2 and Rc, and their co-treatment effect with known tumor necrosis factor(TNF)-α antagonist on TNF-α production in either murine(RAW264.7) or human (U937) macrophages stimulated with lipopolysaccharide(LPS). Rb1 and Rb2 strongly suppressed TNF-α production in RAW 264.7 cells with an IC50 of 56.5 and 27.5μM, respectively, and in differentiated U937 cells with an IC50 of 51.3 and 26.8μM, respectively. The inhibitory activity of Rb1 and Rb2 was significantly increased by pharmacological agents against protein kinase C, protein tyrosine kinase, and protein kinase A, and anti-rheumatoid arthritis drugs, such as chlorophodiesterase (cAMP PDE) inhibitors among cAMP-elevating agents did not change the inhibitory potency of PPDGs. These data suggest that PPDGs may possess potential therapeutic efficacy against TNF-α mediated disease and the therapeutic potency of PPDGs may be enhanced when co-treated with various kinds of known TNF-α antagonists but not with cAMP PDE inhibitors.

목차 (Table of Contents)

• Introduction
• Materials and Methods
• Materials
• Stimulation of TNF-α production in murine and human macrophages
• Statistical analysis
• Results and Discussion
• Effect of PPDGs(Rb1, Rb2 and Rc) and PPTGs(Re and Rg1) on THF-α production by LPS-stimulated murine macrophage cell line RAW264.7
• Cotreatment effects of PPDGs and known TNF-α inhibitors