Temporal Changes of the Calcium-binding Proteins in the Medial Vestibular Nucleus following Unilateral Labyrinthectomy in Rats

다국어 초록 (Multilingual Abstract)

Calcium (Ca²+) is an intracellular second messenger associated with neuronal plasticity of the central
nervous system. The calcium-binding proteins regulate the Ca²+-mediated signals in the cytoplasm
and buffer the calcium concentration. This study examined temporal changes of three calcium-binding
proteins (calretinin, calbindin and parvalbumin) in the medial vestibular nucleus (MVN) during vestibular
compensation after unilateral labyrinthectomy (UL) in rats. Rats underwent UL, and the
changes in the expression of these proteins at 2, 6, 12, 24, 48, and 72 h were examined by immunofluorescence
staining. The expression levels of all three proteins increased immediately after UL and
returned to the control level by 48 h. However, the level of calretinin showed changes different from
the other two proteins, being expressed at significantly higher level in the contralateral MVN than
in the ipsilateral MVN 2 h after UL, whereas the other two proteins showed similar expression levels
in both the ipsilateral and contralateral MVN. These results suggest that the calcium binding proteins
have some protective activity against the increased Ca²+ levels in the MVN. In particular, calretinin
might be more responsive to neuronal activity than calbindin or parvalbumin.

다국어 초록 (Multilingual Abstract)

Calcium (Ca²+) is an intracellular second messenger associated with neuronal plasticity of the central
nervous system. The calcium-binding proteins regulate the Ca²+-mediated signals in the cytoplasm
and buffer the calcium concentration. This study examined temporal changes of three calcium-binding
proteins (calretinin, calbindin and parvalbumin) in the medial vestibular nucleus (MVN) during vestibular
compensation after unilateral labyrinthectomy (UL) in rats. Rats underwent UL, and the
changes in the expression of these proteins at 2, 6, 12, 24, 48, and 72 h were examined by immunofluorescence
staining. The expression levels of all three proteins increased immediately after UL and
returned to the control level by 48 h. However, the level of calretinin showed changes different from
the other two proteins, being expressed at significantly higher level in the contralateral MVN than
in the ipsilateral MVN 2 h after UL, whereas the other two proteins showed similar expression levels
in both the ipsilateral and contralateral MVN. These results suggest that the calcium binding proteins
have some protective activity against the increased Ca²+ levels in the MVN. In particular, calretinin
might be more responsive to neuronal activity than calbindin or parvalbumin.

참고문헌 (Reference)

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