산모 말초 혈액으로부터 태아 세포의 분리 = Isolation of Fetal Cells from Perlpheral Blood of Pregnant Women

다국어 초록 (Multilingual Abstract)

We synthesized rabbit antisera to the enzyme, human type Ⅰ 3-HSD(3-hydroxy-5-ene steroid dehydrogenase, EC 1.1.1.145) that is the key enzyme in the stroidogenesis and membrane-bounding enzyme of trophoblasts, by oligopeptide coupling method. This anti-3-HSD antisera reacts with 43kDa protein in human placental lysate by SDS-PAGE and Western blot, and the syncytiotrophoblasts and cytotrophoblasts from placental villi are stained positively with this antisera by immunohistochemistry (published in Bulletin of Life Science and Biotechnology 1995;3:68).
Fetal trophoblast cells were isolated from peripheral blood of pregnant women by the immune-bead method using anti-3-HSD antibody and Dynabeads M-280(Dynal A.S. Oslo Norway). Isolated cells were observed directly by inverted microscope and observed with Pap stain by light microscope. Isolated trophoblast cells were adherent to Dynabeads M-280 and exhibited the typical morphology of syncytiotrophoblast cells.
We mentioned previously polymerase chain reaction to the Y-chromosome containing gene, DYZ1, SRY, and AMGL is the easy method in sex-determination(published in Kor J Obstet Gynecol 1997;40:1412). For the purpose of fetal cell identification, fetal cells were isolated from peripheral blood of five women who were pregnant with male fetus ascertained by karyotyping of amnionic cells and ultrasonography. Sex-determination of the isolated fetal cells was performed by PCR method. In all cases(5/5, 100%), maleness was confirmed. According to our results, it was concluded that isolation of these fetall cells by immune beads method could allow noninvasive diagnosis of wide range of inherited disorders.