Paraquat가 Arachidonate Lipoxygenase의 대사에 미치는 영향 = The Effects of Paraquat on Arachidonate Lipoxygenase Metabolism

다국어 초록 (Multilingual Abstract)

Using mixed incubation of cultured endothelial cells, cultured fibroblasts, neutrophils activated with PMA and paraquat, the production of superoxide anion, H₂0₂ and lipoxygenase metabolites (5-HETE and 12-HETE) of arachidonate was estimated.
The results was as follows:
1. Neutrophils activated with PMA was produced superoxide anion, H₂0₂ and lipoxygenase metabolites (5-HETE and 12-HETE) of arachidonate.
2. Fibroblasts did not alter the production of superoxide anion and H₂0₂ by neutrophils, it was markedly reduced by mixed incubation with endothelial cells.
3. Mixed incubation with endothelial cells significantly augmented the production of 5 or 1.2-HETEs, but fibroblasts did not.
4. Using mixed incubation of endothelial cells, fibroblasts, neutrophils and paraquat (50㎍/ml and 100㎍/ml), the production of superoxide anion, H₂0₂ and HETEs was significantly increased on both cells at low concentration (50㎍/ml), but markedly reduced at high concentration (100㎍/ml).
5. Paraquat showed concentration dependent effects on arachidonate lipoxygenase metabolism.