Morphological features and lipopolysaccharide attachment of coliphages specific to Escherichia coli O157:H7 and to a broad range of E. coli hosts

다국어 초록 (Multilingual Abstract)

The objective of the present study was to analyze host-phage adsorption of bacteriophages infecting Escherichia coli O157:H7 and the other E. coli strains. Out of 55 coliphage strains, we selected seven coliphages infectious only to 23 E. coli O157 and seven other coliphages of broad specificity to E. coli O157:H7 and other 61 E. coli. Escherichia coli O157-specific phages and the broadly specific phages all belonged to the Siphoviridae and Myoviridae family, respectively. Escherichia coli O157-specific phages infected E. coli O157:H7, but not E. coli O157:H7△rfaL, deletion mutant of O-antigen ligase gene for lipopolysaccharide. Five coliphages among the broadly specific phages infected E. coli O103, but not E. coli O103△rfaG, deletion mutant of the glycosyltransferase gene. E. coli O157:H7-specific phages among Siphoviridae recognized O-antigen of E. coli O157, but the broadly specific coliphages of Myoviridae may recognize O-antigen and/or a part of the lipopolysaccharide core as an adsorption site in various E. coli. The receptor of the two coliphage groups interacts with some part of lipopolysaccharide, and the tail morphology of the coliphages may be related to their adsorption to and recognition of a different part of lipopolysaccharide. In particular, specificity of E. coli O157:H7-specific phages carrying the long tail of Siphoviridae for O-antigen as a receptor seems to be high.

참고문헌 (Reference)

1 Bebeacua C, "Visualizing a complete Siphoviridae member by single-particle electron microscopy: the structure of lactococcal phage TP901-1" 87 : 1061-1068, 2013

2 Pate JL, "Two flagellotropic phages and one pilus-specific phage active against Asticcacaulis biprosthecum" 94 : 24-37, 1979

3 Lodge JK, "Transposon Tn5 target specificity: preference for insertion at G/C pairs" 120 : 645-650, 1988

4 Goryshin IY, "Tn5/IS50 target recognition" 95 : 10716-10721, 1998

5 Riede I, "The receptor specificity of bacteriophages can be determined by a tail fiber modifying protein" 4 : 2343-2346, 1985

6 Chang V, "The effect of lipopolysaccharide core structure defects on transformation efficiency in isogenic Escherichia coli BW25113 rfaG, rfaP, and rfaC mutants" 14 : 101-107, 2010

7 Yu F, "Roles of lipopolysaccharide and outer membrane protein OmpC of Escherichia coli K-12 in the receptor function for bacteriophage T4" 151 : 718-722, 1982

8 Vinga I, "Role of bacteriophage SPP1 tail spike protein gp21 on host cell receptor binding and trigger of phage DNA ejection" 83 : 289-303, 2012

9 Shin H, "Receptor diversity and host interaction of bacteriophages infecting Salmonella enterica serovar Typhimurium" 7 : e43392-, 2012

10 Yethon JA, "Mutation of the lipopolysaccharide core glycosyltransferase encoded by waaG destabilizes the outer membrane of Escherichia coli by interfering with core phosphorylation" 182 : 5620-5623, 2000

11 Lee JE, "Molecular characterization of bacteriophages for microbial source tracking in Korea" 75 : 7107-7114, 2009

12 Vinga I, "Modern bacteriophage biology and biotechnology" Research Signpost, INIST-CNRS 165-205, 2006

13 Mudhakir D, "Learning from the viral journey: how to enter cells and how to overcome intracellular barriers to reach the nucleus" 11 : 65-77, 2009

14 Randall-Hazelbauer L, "Isolation of the bacteriophage lambda receptor from Escherichia coli" 116 : 1436-1446, 1973

15 Chatterjee S, "Interaction of bacteriophage l with its E. coli receptor" 4 : 3162-3178, 2012

16 Lindberg AA, "Interaction between bacteriophage Sf6 and Shigella flexner" 27 : 38-44, 1978

17 Green B, "Insertion site preference of Mu, Tn5, and Tn7 transposons" 3 : 3-7, 2012

18 Traurig M, "Identification of bacteriophage K20 binding regions of OmpF and lipopolysaccharide in Escherichia coli K-12" 181 : 101-108, 1999

19 Genevaux P, "Identification of Tn10 insertions in the rfaG, rfaP, and galU genes involved in lipopolysaccharide core biosynthesis that affect Escherichia coli adhesion" 172 : 1-8, 1999

20 Parker CT, "Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12" 174 : 930-934, 1992

21 Choi Y, "Identification and characterization of a novel flagellum-dependent Salmonella-infecting bacteriophage, iEPS5" 79 : 4829-4837, 2013

22 Santos SB, "Genomic and proteomic characterization of the broad-host-range Salmonella phage PVP-SE1: creation of a new phage genus" 85 : 11265-11273, 2011

23 Austin EA, "Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli K-12: insertion mutagenesis of the rfa locus" 172 : 5312-5325, 1990

24 Wu N, "Enhancing DNA electrotransformation efficiency in Escherichia coli DH10B electrocompetent cells" 13 : 21-22, 2010

25 Morita M, "Characterization of a virulent bacteriophage specific for Escherichia coli O157: H7 and analysis of its cellular receptor and two tail fiber genes" 211 : 77-83, 2002

26 Kim EJ, "Characterization and infection receptor analysis of bacteriophages for Escherichia coli O157:H7 and non-O157 shiga toxin-producing E. coli" Gachon University 2015

27 Li S, "Characterization and genome sequencing of a novel coliphage isolated from engineered Escherichia coli" 53 : 211-220, 2010

28 Park M, "Characterization and comparative genomic analysis of a novel bacteriophage, SFP10, simultaneously inhibiting both Salmonella enterica and Escherichia coli O157:H7" 78 : 58-69, 2012

29 Lee YD, "Characteristics of coliphage ECP4 and potential use as a sanitizing agent for biocontrol of Escherichia coli O157: H7" 34 : 255-260, 2013

30 Ackermann HW, "Bacteriophages" Humana Press 113-126, 2009

31 Ackermann HW, "Bacteriophages" Humana Press 127-140, 2009

32 Kutter E, "Bacteriophages" Humana Press 141-149, 2009

33 Rakhuba DV, "Bacteriophage receptors, mechanisms of phage adsorption and penetration into host cell" 59 : 145-155, 2010

34 Friedman DI, "Bacteriophage lambda: alive and well and still doing its thing" 4 : 201-207, 2001

35 Reyes-Cortes R, "A novel strategy to isolate cell-envelope mutants resistant to phage infection: bacteriophage mEp213 requires lipopolysaccharides in addition to FhuA to enter Escherichia coli K-12" 158 : 3063-3071, 2012

36 Veesler D, "A common evolutionary origin for tailed-bacteriophage functional modules and bacterial machineries" 75 : 423-433, 2011