Antimicrobial activity of tea tree oil nanoparticles against American and European foulbrood diseases agents

다국어 초록 (Multilingual Abstract)

Paenibacillus larvae and Melissococcus plutonius are the primary bacterial pathogens of honeybees and the causativeagents of American and European foulbrood disease (AFB and EFB) respectively. Such diseases have beengaining importance since there are few therapeutic options beyond the reporting of microorganisms resistantto conventional antibiotics. Due to the inefficiency and/or lowefficacy of some antibiotics, researches with nanotechnologyrepresent, possibly, new therapeutic strategies. Nanostructured drugs have presented some advantagesover the conventional medicines, such as slow, gradual and controlled release, increased bioavailability,and reduced side-effects, among others. In this study, in vitro antimicrobial activity of tea tree oil (TTO) nanoparticlesagainst Paenibacillus species, including P. larvae and M. plutonius strains was evaluated. Minimal inhibitoryconcentration (MIC) in Mueller–Hinton or KSBHI broth by the microdilution method was assessed. TTO registeredMIC values of 0.18–6.25%, while the MIC values obtained for the TTO nanoparticle were of 0.01–0.93%.
The possible toxic effect of TTO and TTO nanoparticle has been assessed by the spraying application method inthe concentrations higher than the MICs. Bee mortality was evident only in treatment with TTO and the TTOnanoparticles show no toxic effects after 7 days of observation. Our results showed for the first time that TTOnanoencapsulation presented a high activity against Paenibacillus species and M. plutonius strains showing thatthe use of nanotechnology may represent one alternative way for the treatment or prevention of AFB and EFB.

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