Grape extracts suppress the formation of preneoplastic foci and activity of fatty acid synthase in rat liver

다국어 초록 (Multilingual Abstract)

This study was conducted to examine the efects of dietary grape extracts on preneoplastic foci for-mation in rat hepatocarcinogenesis, and related hepatic enzymes. Male Sprague-Dawley rats were fed basal diet or grape diet containing 15% con-centrated grape extracts (68 bricks). The grape diet groups were divided into whole-period grape diet group (DEN-GW; grape diet group fed throughout group (DEN-GP; grape diet group fed from post initiation stage) acording to the starting time point of the grape diet. Hepatocarcinogenesis was in-duced by diethylnitrosamine (DEN; 200 mg/kg bw) and 2/3 partial hepatectomy (DEN-B; DEN-treated basal diet group, DEN-GW, and DEN-GP groups), while the control group treated with saline and sham operation (Control group). The formation of pla-cental glutathione (GSH) S-transferase positive (GST-P+) foci in DEN-GW group was moderately GP group. Thiobarbituric acid reactive substances content of DEN-GW group was significantly lower than that of DEN-B group. The activity of faty acid synthase (FAS) in the grape diet groups was de-creased about 1/2 of the DEN-B group. The con-tent of GSH and GSH peroxidase activity were in-creased by carcinogen treatment, but not modul-ated by grape diet. The activities of GSH S-trans-ferase, p-nitrophenol hydroxylase, and catalase were not affected by diet or treatment. Conclu-sively, the grape diet-induced reduction of FAS tisues, might be one of the contributing mecha-nisms of hepatic cancer prevention.