Candida tropicalis IFO-0589로 부터 구연산 生成 變異株 誘導 = Induction of Citric Acid-Producing Mutants from the Strain Candida tropicalis IFO-0589

다국어 초록 (Multilingual Abstract)

The strain Candida tropicalis IFO-0589 was treated with N-methyl-N’-nitro-N-nitrosoguanidine in order to obtain the most potent citrate-producing mutants. Three mutants 601, 1202 and 1205 were induced and found to be superior and inferior in citrate and d-isocitrate producibilities, respectively, to other strains. When those mutants and parent were cultured in a basal medium containing 10% glucose at 3O℃ for 168 hrs. using a reciprocatory shaker (50mm stroke amplitude), mutant 601 and 1202 were superior to other strains in citrate and cell producibilities, respectively and mutant 1205 was inferior to other strains in d-isocitrate producibility. There was little difference in morphology between those mutants and parent.