Different isolates of Alternanthera mosaic virus (AltMV; Potexvirus), including four infectious clones derived from AltMV-SP, induce distinct systemic symptoms in Nicotiana benthamiana. Virus accumulation was enhanced at 15<SUP>o</SUP>C co...
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https://www.riss.kr/link?id=A107633789
Lim, H.S. ; Nam, J. ; Seo, E.Y. ; Nam, M. ; Vaira, A.M. ; Bae, H. ; Jang, C.Y. ; Lee, C.H. ; Kim, H.G. ; Roh, M. ; Hammond, J.
2014
-
SCI,SCIE,SCOPUS
학술저널
264-278(15쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
Different isolates of Alternanthera mosaic virus (AltMV; Potexvirus), including four infectious clones derived from AltMV-SP, induce distinct systemic symptoms in Nicotiana benthamiana. Virus accumulation was enhanced at 15<SUP>o</SUP>C co...
Different isolates of Alternanthera mosaic virus (AltMV; Potexvirus), including four infectious clones derived from AltMV-SP, induce distinct systemic symptoms in Nicotiana benthamiana. Virus accumulation was enhanced at 15<SUP>o</SUP>C compared to 25<SUP>o</SUP>C; severe clone AltMV 3-7 induced systemic necrosis (SN) and plant death at 15<SUP>o</SUP>C. No interaction with potexvirus resistance gene Rx was detected, although SN was ablated by silencing of SGT1, as for other cases of potexvirus-induced necrosis. Substitution of AltMV 3-7 coat protein (CP<SUB>SP</SUB>) with that from AltMV-Po (CP<SUB>Po</SUB>) eliminated SN at 15<SUP>o</SUP>C, and ameliorated symptoms in Alternanthera dentata and soybean. Substitution of only two residues from CP<SUB>Po</SUB> [either MN(13,14)ID or LA(76,77)IS] efficiently ablated SN in N. benthamiana. CP<SUB>SP</SUB> but not CP<SUB>Po</SUB> interacted with Arabidopsis boron transporter protein AtBOR1 by yeast two-hybrid assay; N. benthamiana homolog NbBOR1 interacted more strongly with CP<SUB>SP</SUB> than CP<SUB>Po</SUB> in bimolecular fluorescence complementation, and may affect recognition of CP as an elicitor of SN.