Penicillium verruculosum 으로부터 Celobiohydrolase 의 정제 및 특성에 관한 연구 = Purification and Characterization of Cellobiohydrolase from Penicillium verrucuiosum

다국어 초록 (Multilingual Abstract)

Three cellobiohydrolase I, II and III, from culture solution of Penicillum verruculosum grown in liquid media containing cellobiose octaacetate or KC-flock, as the sole carbon source has been extensively purified and characterized. Molecular weights of the purified enzymes, I, II and III, were estimated as 60,000, 66,000 and 76,000 on 10% polyacrylamide gel containing sodium dodecyl sulfate and have 8.6, 4.2 and 8.5% of carbohydrate contents, respectively. The three enzymes showed hydrolytic activities towards crystalline celluloses such as Avicel, cotton and filter paper and soluble cellulose such as carboxymethylcellulose. Specific activities of cellobiohydrolase, I, II and III against Avicel as a substrate were 0.07, 0.10 and 0.07 unit per mg. of protein, respectively. The optimum pHs and temperatures of three enzymes were 5.0 and 50℃. All enzyme produced extensively cellobiose from Avicel, and the enzymatic hydrolysises of p-Nitrophenyl-β-D-cellobioside were inhibited by cellobiose, but not by D-glucose.