Evaluation of Morphological Changes in Degenerative Cartilage Using 3-D Optical Coherence Tomography

다국어 초록 (Multilingual Abstract)

Optical Coherence Tomography (OCT) is an important noninvasive medical imaging technique
that can reveal subsurface structures of biological tissue. OCT has demonstrated a good correlation
with histology in sufficient resolution to identify morphological changes in articular cartilage to
differentiate normal through progressive stages of degenerative joint disease. Current OCT systems
provide individual cross-sectional images that are representative of the tissue directly under the
scanning beam, but they may not fully demonstrate the degree of degeneration occurring within
a region of a joint surface. For a full understanding of the nature and degree of cartilage degeneration
within a joint, multiple OCT images must be obtained and an overall assessment of the
joint surmised from multiple individual images. This study presents frequency domain threedimensional
(3-D) OCT imaging of degenerative joint cartilage extracted from bovine knees. The
3-D OCT imaging of articular cartilage enables the assembly of 126 individual, adjacent, rapid
scanned OCT images into a full 3-D image representation of the tissue scanned, or these may be
viewed in a progression of successive individual two-dimensional (2-D) OCT images arranged in
3-D orientation. A fiber-based frequency domain OCT system that provides cross-sectional images
was used to acquire 126 successive adjacent images for a sample volume of 6×3.2×2.5 mm3. The
axial resolution was 8 μm in air. The 3-D OCT was able to demonstrate surface topography and
subsurface disruption of articular cartilage consistent with the gross image as well as with histological
cross-sections of the specimen. The 3-D OCT volumetric imaging of articular cartilage provides
an enhanced appreciation and better understanding of regional degenerative joint disease than
may be realized by individual 2-D OCT sectional images.

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