Analysis and comparison of cloning methods for the preparation of repetitive polypeptides

다국어 초록 (Multilingual Abstract)

Repetitive polypeptides, defined as a protein consisting primarily of tandemly repeated blocks of amino
acid sequence, are widely used biomaterials. These repetitive polypeptides can be used in diverse biological fields including
tissue engineering scaffolds, drug delivery systems, biomaterials, and DNA separation systems. The physical/
chemical properties of the repetitive polypeptides can be improved by changing the composition of the repeated amino
acid sequence. In this study, we introduced genetic methods for the production of repetitive polypeptides. By using
recursive directional ligation (RDL) and controlled cloning method (CCM), multimerized genes were cloned and identified.
Also, we compared the characteristics of recursive directional ligation (RDL) with those of controlled cloning
method (CCM).

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