Characterization of high temperature-tolerant strains of Pyropia yezoensis

다국어 초록 (Multilingual Abstract)

High-temperature stress related to global warming reduces the growth and productivity of seaweeds. Thus, the development of new strains is urgently required for maintaining or even enhancing the productivity of useful seaweeds such as red alga Pyropia yezoenesis in an increasingly warmer sea environment. To develop competitive high-temperature-tolerant strains of P. yezoensis (Sugwawon no. 104), we screened libraries of gamma-irradiated strains and identified high-temperature-resistant (HTR) mutants. Our results showed that HTR-1 and HTR-2 grew well at higher temperatures that inhibited the growth of the wild-type strain. The efficiency of conchosporangium maturation and conchospore release of HTR-1 was similar to or higher than the wild-type strain. Moreover, thallus growth, pigment content, photosynthetic efficiency, and monospore release from the growing thallus in HTR-1 could be maintained even at high temperature. Taken together, our data demonstrate that HTR-1 may be suitable for industrial cultivation at sea, even at elevated temperatures.

참고문헌 (Reference)

1 김승오, "김속식물 3종의 생장과 단포자방출에 미치는 해양심층수의 영향" 한국해양학회 17 (17): 1-8, 2012

2 Choi S, "Transcriptome sequencing and comparative analysis of the gametophyte thalli of Pyropia tenera under normal and high temperature conditions" 25 (25): 1237-1246, 2013

3 Hongfeng G, "The variation in the contents of phycobiliproteins from Porphyra haitanensis collected in different growing stages" 24 : 645-648, 1993

4 Fu CH, "The releasing and high temperature resistance of conchospores from an improved strain in Porphyra yezoensis Ueda (Bangiales, Rhodophyta)" 42 : 460-466, 2011

5 Yan XH, "Selection and characterization of a high-temperature tolerant strain of Porphyra haitanensis Chang et Zheng (Bangiales, Rhodophyta)" 22 (22): 511-516, 2010

6 Wang HZ, "Selection and characterization of a high-temperature resistant strain of Porphyra yezoensis Ueda (Bangiales, Rhodophyta)" 2 : 363-369, 2012

7 Ashraf M, "Photosynthesis under stressful environments : an overview" 51 (51): 163-190, 2013

8 Chen SS, "Isolation and characterization of an improved strain of Porphyra chauhanii (Bangiales, Rhodophyta) with high-temperature resistance" 28 (28): 3031-3041, 2016

9 Ding H, "Isolation and characterization of a heat-resistant strain with high yield of Pyropia yezoensis Ueda (Bangiales, Rhodophyta)" 1 : 24-33, 2016

10 Park EJ, "Genetic polymorphism within Porphyrayezoensis (Bangiales, Rhodophyta) and related species from Japan and Korea detected by cleaved amplified polymorphic sequence analysis" 42 (42): 29-40, 2007

11 Park EJ, "Genetic polymorphism within Porphyra yezoensisrelated species (Bangiales, Rhodophyta) from Japan and Korea detected by CAPS analysis CAPS" Nat’1. Univ. Hokkaido 2006

12 Nikaido I, "Generation of 10,154 expressed sequence tags from a leafy gametophyte of a marine red alga, Porphyra yezoensis" 7 (7): 223-227, 2000

13 Wang SJ, "Gamma-rays induction of mutation in conchocelis of Porphyra yezoensis" 18 (18): 47-53, 2000

14 Zhang BL, "Evaluation of an improved strain of Porphyra yezoensis Ueda (Bangiales, Rhodophyta) with hightemperature tolerance" 23 (23): 841-847, 2011

15 Porra RJ, "Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents : verification of the concentration of chlorophyll standards by atomic absorption spectroscopy" 975 (975): 384-394, 1989

16 Kuwano K, "Cryopreservation of clonal gametophytic thalli of Porphyra(Rhodophyta)" 116 : 117-124, 1996

17 Miura A, "Cross breeding in cultivars of Porphyra yezoensis Ueda(Bangiales, Rhodophyta). A preliminary report" 4 : 207-211, 1989

18 Wang WX, "Biotechnology of plant osmotic stress tolerance physiological and molecular considerations" 560 : 285-292, 2000