Application of the Coral Health Chart to Determine Bleaching Status of Acropora downingi in a Subtropical Coral Reef

다국어 초록 (Multilingual Abstract)

The ‘Coral Health Chart’ has become a popular tool for monitoring coral bleaching worldwide. The scleractinian coral Acropora downingi (Wallace 1999) is highly vulnerable to temperature anomalies in the Persian Gulf. Our study tested the reliability of Coral Health Chart scores for the assessment of bleaching-related changes in the mitotic index (MI) and density of zooxanthellae cells in A. downingi in Qeshm Island, the Persian Gulf. The results revealed that, at least under severe conditions, it can be used as an effective proxy for detecting changes in the density of normal, transparent, or degraded zooxanthellae and MI. However, its ability to discern changes in pigment concentration and total zooxanthellae density should be viewed with some caution in the Gulf region, probably because the high levels of environmental variability in this region result in inherent variations in the characteristics of zooxanthellae among “healthy” looking corals.

참고문헌 (Reference)

1 Cruz ICS, "White but not bleached: photophysiological evidence from white Montastraea cavernosa reveals potential overestimation of coral bleaching" 162 : 889-899, 2015

2 Williams E, "The world-wide coral reef bleaching cycle and related sources of coral mortality" 335 : 1-71, 1990

3 Stimson J, "The temporal pattern and rate of release of zooxanthellae from the reef coral Pocillopora damicornis (Linnaeus) under nitrogen-enrichment and control conditions" 153 (153): 63-74, 1991

4 Putnam HM, "The physiological response of reef corals to diel fluctuations in seawater temperature" 396 : 216-223, 2011

5 Muscatine L, "The effect of external nutrient resources on the population dynamics of zooxanthellae in a reef coral" 236 : 311-324, 1989

6 Fagoonee I, "The dynamics of zooxanthellae populations: a long-term study in the field" 283 : 843-845, 1999

7 De’ath G, "The 27–year decline of coral cover on the Great Barrier Reef and its causes" 109 : 17995-17999, 2012

8 Wilkerson FP, "Temporal patterns of cell division in natural populations of endosymbiotic algae" 28 : 1009-1014, 1983

9 Jones RJ, "Temperature-induced bleaching of corals begins with impairment of the CO2 fixation mechanism in zooxanthellae" 21 : 1219-1230, 1998

10 Dimond J, "Symbiosis regulation in a facultatively symbiotic temperate coral: zooxanthellae division and expulsion" 27 : 601-604, 2008

11 Baker AC, "Symbiosis ecology of reef-building corals" University of Miami 1999

12 Cohen J, "Statistical power analysis for the behavioral sciences" Routledge 400-, 1988

13 Wake B, "Snapshot: snow white coral" 6 : 439-439, 2016

14 Muller-Parker G, "Seasonal variation in light-shade adaptation of natural populations of the symbiotic sea anemone Aiptasia pulchella (Carlgren, 1943) in Hawaii" 112 : 165-183, 1987

15 Fitt WK, "Seasonal patterns of tissue biomass and densities of symbiotic dinoflagellates in reef corals and relation to coral bleaching" 45 : 677-685, 2000

16 Hughes TP, "Rising to the challenge of sustaining coral reef resilience" 25 : 633-642, 2010

17 Mumby PJ, "Remote sensing of coral reefs and their physical environment" 48 : 219-228, 2004

18 Bhagooli R, "Release of zooxanthellae with intact photosynthetic activity by the coral Galaxea fascicularis in response to high temperature stress" 145 : 329-337, 2004

19 Chow MH, "Quantifying the degree of coral bleaching using digital photographic technique" 479 : 60-68, 2016

20 Dunn SR, "Programmed cell death and cell necrosis activity during hyperthermic stress-induced bleaching of the symbiotic sea anemone Aiptasia sp" 272 : 29-53, 2002

21 Marsh JA, "Primary productivity of reef-building calcareous red algae" 51 : 255-263, 1970

22 Shenkar N, "Population dynamics of zooxanthellaee during a bacterial bleaching event" 25 : 223-227, 2006

23 Falkowski PG, "Population control in symbiotic corals" 43 : 606-611, 1993

24 Reimer AA, "Observations on the relationships between several species of tropical zoanthids (Zoanthidea, coelenterata)and their Zooxanthellae" 7 : 207-214, 1971

25 Ladrière O, "Natural spatial variability of algal endosymbiont density in the coral Acropora globiceps: a small-scale approach along environmental gradients around Moorea (French Polynesia)" 94 : 65-74, 2014

26 Fadlallah YH, "Mortality of shallow reef corals in the western Arabian Gulf following aerial exposure in winter" 14 (14): 99-107, 1995

27 Siebeck UE, "Monitoring coral bleaching using a color reference card" 25 : 453-460, 2006

28 Pochon X, "Molecular phylogeny, evolutionary rates, and divergence timing of the symbiotic dinoflagellate genus Symbiodinium" 38 : 20-30, 2006

29 Lisa Fujise, "Moderate Thermal Stress Causes Active and Immediate Expulsion of Photosynthetically Damaged Zooxanthellae (Symbiodinium) from Corals" Public Library of Science (PLoS) 9 (9): e114321-, 2014

30 Wilkerson, FP, "Mitotic index and size of symbiotic algae in Caribbean reef corals" 7 (7): 29-36, 1988

31 Hill J, "Methods for ecological monitoring of coral reefs: a resource for managers" Australian Institute of Marine Science (AIMS) 117-, 2004

32 Kavousi J, "Mass coral bleaching in the northern Persian Gulf, 2012" 78 (78): 397-404, 2014

33 Brown BE, "Marine ecology: bleaching patterns in reef corals" 404 : 142-143, 2000

34 Sheppard C, "Marine ecology of the Arabian region: patterns and processes in extreme tropical environments" Academic Press 359-, 1992

35 LaJeunesse TC, "Longstanding environmental conditions, geographic isolation and host–symbiont specificity influence the relative ecological dominance and genetic diversification of coral endosymbionts in the genus Symbiodinium" 37 (37): 785-800, 2010

36 Arar EJ, "In vitro determination of chlorophylls a, b, c1, +c2and pheopigments in marine and freshwater algae by visible spectrophotometry"

37 Glynn PW, "Global ecological consequences of the 1982–83 El Niño-Southern Oscillation" Elsevier 55-126, 1990

38 Jeong HJ, "Genetics and morphology characterize the dinoflagellate Symbiodinium voratum, (Dinophyceae) as the sole representative of Symbiodinium clade E" 61 (61): 75-94, 2014

39 Fujise L, "Expulsion of zooxanthellae (Symbiodinium) from several species of scleractinian corals: comparison under non-stress conditions and thermal stress conditions" 15 : 29-36, 2013

40 Lesser MP, "Exposure to solar radiation increases damage to both host tissues and algal symbionts of corals during thermal stress" 23 : 367-377, 2004

41 Mise T, "Degradation of zooxanthellae in the coral Acropora nasuta during bleaching" 5 : 33-39, 2003

42 Titlyanov EA, "Degradation of zooxanthellae and regulation of their density in hermatypic corals" 139 : 167-178, 1996

43 Rudolph P, "Correlation between mitotic and Ki-67 labeling indices in paraffin-embedded carcinoma specimens" 29 (29): 1216-1222, 1998

44 Marshall NJ, "CoralWatch: education, monitoring, and sustainability through citizen science" 10 : 332-334, 2012

45 Riegl BM, "Coral reefs of the gulf: adaptation to climatic extremes in the world’s hottest sea" Springer 379-, 2012

46 Goreau TJ, "Coral bleaching and ocean hot spots" 23 : 176-180, 1994

47 Bolouki M, "Comparison of symbiotic algae densities in coral comparison of symbiotic algae densities in coral" 4 : 45-51, 2013

48 Brown BE, "Comparative measurements of mitotic index in zooxanthellae from a symbiotic cnidarian subject to temperature increase" 158 (158): 179-188, 1992

49 Hoegh-Guldberg O, "Climate change, coral bleaching and the future of the world's coral reefs" 50 : 839-866, 1999

50 Kuroki T, "Changes in zooxanthellae characteristics in the coral Stylophora pistillata during the 1998 bleaching event" 1999 : 97-101, 1999

51 Thompson JD, "CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice" 22 : 4673-4680, 1994

52 Strychar KB, "Bleaching as a pathogenic response in scleractinian corals, evidenced by high concentrations of apoptotic and necrotic zooxanthellae" 304 : 99-121, 2004

53 Zar JH, "Biostatistical analysis" Prentice Hall 929-, 1998

54 Montano S, "Assessing coral bleaching and recovery with a color reference card in Watamu Marine Park, Kenya" 655 : 99-108, 2010

55 John Everett Parkinson, "A citizen science approach to monitoring bleaching in the zoantharian Palythoa tuberculosa" PeerJ 4 : e1815-, 2016