Determination of mutagenic sensitivity to gamma rays in ginseng ( Panax ginseng ) dehiscent seeds, roots, and somatic embryos

다국어 초록 (Multilingual Abstract)

Ginseng ( Panax ginseng ) has a low genetic diversity and a narrow pool of genetic resources. Mutagenesis is one of the mostpowerful methods for inducing genetic variation in this species, but little research has been performed in ginseng. In thisstudy, various tissues, including dehiscent seeds, 1-year-old roots, and somatic embryos were irradiated at diff erent dosesof gamma rays (20–400 Gy and zero dose as a control) to determine the most optimal concentration and tissues for the successfuluse of mutagenesis in ginseng breeding. The results revealed that high gamma doses (> 100 Gy) were detrimentalto all irradiated tissues. A gradual and signifi cant reduction in germination, emergence, and seedling growth were found asgamma irradiation dose increased. The reduction in survival rates and seedling growth by irradiation at serial doses showedthat the LD 50 of ginseng was 20–80 Gy, although the irradiation doses were tissue dependent. Based on our results, theoptimal doses of gamma rays for inducing mutation in ginseng are < 20, 40, and 60–80 Gy for 1-year-old roots, dehiscentseeds, and somatic embryos, respectively. Given the fact that ginseng somatic embryos are less sensitive to gamma rays thanother tissues, the combination of in vitro culture and mutagenesis could be more eff ective than the conventional methodfor mutation breeding in ginseng. These results provide a good basis for radiation sensitivity of ginseng and are useful as aguideline for ginseng mutation breeding.

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