Negative Effects of Water Extracts from Pinus densiflora Sawdust on Mycelium Growth of the Shiitake Mushroom Lentinula edodes

다국어 초록 (Multilingual Abstract)

Extracts from sawdust of Pinus densiflora were showed antifungal activity against Lentinula edodes. It was extracted by
hot water and then successively extracted by n-hexane, ethyl acetate, and methanol. The yields of the n-hexane-soluble, ethyl acetate-
soluble, methanol-soluble and methanol-insoluble fractions of water extracts were 8.2%, 10.6%, 32.0%, and 49.2%, respectively. The
ethyl acetate-soluble fraction showed the greatest antifungal activity against L. edodes: 41.5% inhibition at 1,000 ppm. However, there
were not significant differences of antifungal activities between n-hexane-soluble fraction and methanol-soluble fraction at a concentration
of 1,000 ppm. The hot water extracts showed 23.5% of antifungal activity against L. edodes at a concentration of 1000 ppm.
The four antifungal compounds were separated from ethyl acetate fraction by thin layer chromatography.

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