Transforming Growth Factor-β1이 악성림프종과 백혈병 세포주의 성장, c-myc mRNA 발현, Telomerase 활성에 미치는 영향 = Effect on Cell Growth, c-myc mRNA Expression and Telomerase Activity by Transforming Growth Factor-β1 in Malignant Lymphoma and Leukemia Cell Line

국문 초록 (Abstract)

배경: TGF-β1은 대부분의 상피세포를 포함한 여러 종류 세포의 성장을 억제하는 것으로 알려져 있으나 악성림프종과 백혈병에서 세포 성장에 대한 작용기전은 잘 알려져 있지 않다. c-myc 유전자의 과발현은 세포증식과 세포자멸사를 조절하며 종말체 효소는 암의 발생에 중요한 역할을 한다고 알려져 있다. 이에 본 연구에서는 악성림프종과 백혈병 세포주에서 TGF-β1이 세포성장과 종말체 효소 활성에 미치는 영향과 c-myc 유전자와의 상관관계를 통해 TGF-β1의 작용 기전을 알아보고자 하였다. 방법: Burkitt 림프종 세포주(Jiyoye), T세포 림프종 세포주(H9)와 급성 T림프구성 백혈병 세포주(CCRF- CEM)를 사용하였으며 TGF-β1이 세포성장에 미치는 영향을 평가하기 위하여 MTT법을 이용하였다. TGF-β1을 투여한 후 c-myc 유전자 변화를 관찰하기 위하여 RT-PCR법으로 c-myc RNA의 발현을 측정하였고 종말체 효소 활성은 TRAP (telomeric repeat amplification protocol)을 이용하여 분석하였다. 결과: 세포성장에 대한 영향을 알아본 결과 TGF-β1 (5ng/mL)을 투여한 후 Jiyoye 세포주는 대조군에 비해 시간이 지남에 따라 세포성장이 억제되었다(P＜0.005). c-myc mRNA 발현과 종말체 효소 활성은 TGF-β1으로 처리한 Jiyoye 세포주에서 시간이 지남에 따라 유의하게 억제되었다. 그러나 H9와 CCRF-CEM 세포주에서는 TGF-β1으로 처리한 후 세포성장 억제, c-myc mRNA 감소, 종말체 효소 활성 억제가 관찰되지 않았다. 즉 TGF-β1에 의한 세포성장은 c-myc mRNA 발현, 종말체 효소 활성은 서로 밀접한 상관관계를 보였다.

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