雜種 自植系統을 利用한 벼의 RELP 遺傳子 地圖 作成 = Restriction Fragment Length Polymorphisms of Recombinant Inbred Lines of Rice

다국어 초록 (Multilingual Abstract)

Numerous genetic studies have been focused on the elucidation of the structure of rice genome because of its economic importance in the world as well as its shortest genome size among monocots. Restriction fragment length polymorphism(RFLP) is a leading method to map a genome. It has been proven that recombinant inbred lines(RILs) provide an efficient way of mapping genetic markers. In this study, RFLPs of RILs of the rice were determined as follows. Some 164 F_11 individuals of RILs have been derived from F_2 populations of Milyang23 × Gihobyeo. Genomic DNAs were isolated from both the parents and RIL individuals. The DNAs were restricted by 8 different endonucleases (BamH I , Dra I , EcoR I , HindⅢ, EcoRⅤ, Xba I , Sca I , and Kpn I ). The restriction fragments were resolved on agarose gel(0.9%) for Southern blots. RFLP patterns of the parents and RIL individuals were determined using the cDNA clones of RZ & KCD, and the genomic clones of Pst I-digested RG as probes. The RFLP patterns and the linkage map of the genome were determined with the aid of a computer program, Macintosh Mapmaker 3.0. The map shows 125 genetic markers including 106 RFLP loci(4 KCD, 67 RG, and 35 RZ clones). This molecular map contains 1,196.8 centi-Morgan(cM) with average interval size of 9.57 cM on the framework map. This study could facilitate chromosome walking, map-based cloning, quantitative trait mapping, marker-assisted breeding, and evolutionary studies of the rice.

목차 (Table of Contents)

• I. 서 론
• II. 재료 및 방법
• 1. 재 료
• 2. 방 법
• III. 결과 및 고찰
• 1. 교배친의 RFLP Survey
• 2. Mapping용 RFLP Scoring
• 3. 벼의 유전자 연관 지도 작성