Continuous automated system for the determination of guanine was developed. The system was composed of enzyme reactor, gas-dialyzer and tubular PVC-nonactin membrane ammonium ion-selectiveindicator electrode. The enzyme reactor was constructed to pack...
Continuous automated system for the determination of guanine was developed. The system was composed of enzyme reactor, gas-dialyzer and tubular PVC-nonactin membrane ammonium ion-selectiveindicator electrode. The enzyme reactor was constructed to packed-bed form which filled with glass beads(pore diameter 500 Å, particle size 125 ~ 177 μm), which immobilized enzyme on, in teflon tube(2 mm I.D., 20 cm length). The response characteristics of the indicator electrode was superior to those of ammonia gas-sensing electrode. Guanine in 0.1 M tris-HCl buffer solution could be determined by using this system. The linear response range, response slope and detection limit for the determination of guanine in this buffer solution were found to be 1.0x10^(-3) ~ 1.0x10^(-1) M, 30.69 mV/decade and 5.8x10^(-4) M from the plot of cell emf vs. guanine concentration, respectively.