For the purpose of examining whether prostaglandins exist in plant materials. an attempt was made to develop an analytical method to extract and identify prostaglandins thexefrom. Prostaglandin extracts were obtained by a multistage solvent extraction...
For the purpose of examining whether prostaglandins exist in plant materials. an attempt was made to develop an analytical method to extract and identify prostaglandins thexefrom. Prostaglandin extracts were obtained by a multistage solvent extraction, and they were separated and identified by TLC,HPLC and GC-MS. Main components of prostaglandins were efficiently extracted from the garlics and onions using a variety of complex solvents, In the TLC experiments, it was conformed that extracts of the garlics and onions contained the similar components to PGE₁ and PGA₁. HPLC chromatogram showed that PGE₁ in the garlics and onions existed in very larger quantities than PGA₁. Structure of PGE₁ was identified in the garlics by GC-MS in the MICI mode following the derivatization procedure of prostaglandins.