β-Lactamase (VPA0477) 유전자를 표적으로 Polymerase chain reaction에 의한 장염비브리오(Vibrio parahaemolyticus)의 검출 = Application of the β-lactamase (VPA0477) Gene for the Detection of Vibrio parahaemolyticus by Polymerase Chain Reaction

다국어 초록 (Multilingual Abstract)

In this study, the β-lactamase (VPA0477) gene was used as a new target for the PCR-based detection ofVibrioparahaemolyticus . Primers specific for the β-lactamase (VPA0477) gene of V. parahaemolyticus , were designed and incorporated into a PCR-based assay. The assay was able to specifically detect all of the 191 V. parahaemolyticus strains tested, but did not result in amplification of 39 other Vibrio spp. and nonVibrio spp. strains tested. The detection limit of the assay was 10 CFU of V. parahaemolyticus RIMD2210633 from pure culture broth. The β-lactamase (VPA0477) gene-based assay developed in this study was sensitive and specific, and has great potential for the accurate detection and identification of V. parahaemolyticus in seawater or seafood samples.

참고문헌 (Reference)

1 이근우, "해수에서 분리한 장염비브리오의 항생제 내성 및 암피실린 내성 유전자의 동정" 한국수산과학회 43 (43): 637-641, 2010

2 이남형, "장염비브리오가 보유하는 ß-lactamase (VPA0477)의 유전학적 특성" 한국수산과학회 44 (44): 597-604, 2011

3 김태옥, "완도해역 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus)의 항균제 내성 및 병원성 유전자의 특징" 한국수산과학회 47 (47): 220-226, 2014

4 Pazhani GP, "Trends in the epidemiology of pandemic and non-pandemic strains of Vibrio parahae- molyticus isolated from diarrheal patients in Kolkata, India" 8 : e2815-, 2014

5 Honda T, "The pathogenicity of Vibrio parahae- molyticus and the role of the thermostable direct haemolysin and related haemolysins" 4 : 106-113, 1993

6 Sakazaki R, "Studies on the enteropathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus. 3. Enteropathogenici- ty" 21 : 325-331, 1968

7 Yu S, "Species- specific PCR detection of the food-borne pathogen Vibrio parahaemolyticus using the irgB gene identified by compar- ative genomic analysis" 307 : 65-71, 2010

8 No AR, "Rapid detec- tion of Vibrio parahaemolyticus by PCR targeted to the histone-like nucleoid structure (H-NS) gene and its genetic characterization" 53 : 127-133, 2011

9 Wei S, "Multi- plex PCR assays for the detection of Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio chol- erae with an internal amplification control" 79 : 115-118, 2014

10 Shirai H, "Molecular epidemiological evidence for association of thermostable direct hemolysin (TDH) and TDH-related hemolysin of Vib- rio parahaemolyticus with gastroenteritis" 58 : 3568-3573, 1990

11 Kim YB, "Identification of Vibrio parahae- molyticus strains at the species level by PCR targeted to the toxR gene" 37 : 1173-1177, 1999

12 Makino K, "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae" 361 : 743-749, 2003

13 Liu B, "Development of a real PCR assay for rapid detection of Vibrio parahaemolyticus from seafood" 3 : 204-212, 2012

14 Chen S, "Development of a toxR-based loop- mediated isothermal amplification assay for detecting Vibrio parahaemolyticus" 10 : 41-, 2010

15 Chen M, "Development of O- serogroup specific PCR assay for detection and identification of Vibrio parahaemolyticus" 159 : 122-129, 2012

16 Wang L, "Detection of Vibrio parahaemolyticus in food samples using in situ loop- mediated isothermal amplification method" 515 : 421-425, 2013

17 Peeler JT, "Compendium of Methods for the Microbiological Exami- nation of Food" American Public Health Association 105-120, 1992

18 Kaysner CA, "Compendium of Methods for the Microbiologi- cal Examination of Food" American Public Health Associa- tion 405-420, 2001

19 Kim DG, "Application of the rpoS gene for the detection of Vib- rio anguillarum in flounder and prawn by polymerase chain reaction" 31 : 639-647, 2008

20 Hossain MT, "Application of groEL gene for the species-specific de- tection of Vibrio parahaemolyticus by PCR" 54 : 67-72, 2011