Identification of differentially expressed genes by DNA methylation in cervical cancer = Identification of differentially expressed genes by DNA methylation in cervical cancer

다국어 초록 (Multilingual Abstract)

The objective of this study is to find novel cervical cancer-related genes which are regulated by DNA methylation. We performed the integrated analysis of genome-wide DNA methylation and RNA expression profiles using normal and tumor regions of cervical cancer patient`s tissues. For the validation of epigenetic regulation of selected genes, we treated the cervical cancer cell line, HeLa with demethylating reagent (decitabine: 5-Aza-2`-deoxycytidine) and measured the methylation and expression status by methylation-specific PCR (MS-PCR) and realtime PCR. We identified 19 novel cervical cancer related genes showing differential RNA expression by DNA methylation. Many of selected genes have not been reported in the cervical cancer gene database (CCDB), but showed differential expression in cancer versus normal in the microarray database (GENT). Among candidate genes, the epigenetic regulation of three genes, CAMK2N1, ALDH1A3 and PPP1R3C, was validated in cervical cancer cell line The results indicate that expression of CAMK2N1, ALDH1A3 and PPP1R3C genes are suppressed in cervical cancers by DNA methylation.