Hair Growth Promotion Efficacies of Ethanolic Extracts of Ribes fasciculatum and Callicarpa japonica in UVB-irradiated Human Dermal Papilla Cells

다국어 초록 (Multilingual Abstract)

The purpose of this study was to examine the hair growth promotion efficacies of ethanolic extracts of Ribes fasciculatum (RFEE) and Callicarpa japonica (CJEE) in 20 mJ/cm2 UVB-irradiated human dermal papilla cells (hDPCs) by measuring Wnt-3a, Wnt-5a, extracellular-signal-regulated kinase (ERK), Akt, and β-catenin. In the quantitative RT-PCR assay, treatment with 25 μg/mL RFEE and CJEE, and 1 μM MXD resulted in increased expression of Wnt-3a mRNA by 74.4% (p < 0.01), 211.4% (p < 0.001), and 158.7% (p < 0.01), respectively, and increased expression of Wnt-5a mRNA by 53.1% (p < 0.001), 253.5% (p < 0.001), and 170.2% (p < 0.001), respectively, compared to the control. In the western blotting assay, treatment with 25 μg/mL CJEE and 1 μM MXD resulted in increased expression of phosphorylated ERK-1/2 (p-ERK-1/2) protein by 31.9% (p < 0.001) and 45.4% (p < 0.001), respectively, and increased expression of phosphorylated Akt (p-Akt) protein by 29.9% (p < 0.01) and 11.3% (p > 0.05), respectively, compared to the control. In the β-catenin assay, treatment with 25 μg/mL RFEE and CJEE, and 1 μM MXD resulted in increased production of β-catenin by 17.5% (p > 0.05), 39.2% (p < 0.01), and 86.7% (p < 0.001), respectively, compared to the control.