Functional Connectivity Map of Retinal Ganglion Cells for Retinal Prosthesis

다국어 초록 (Multilingual Abstract)

Retinal prostheses are being developed to restore vision for the blind with retinal diseases such as retinitis pigmentosa (RP) or age-related macular degeneration (AMD). Among the many issues for prosthesis development, stimulation encoding strategy is one of the most essential electrophysiological issues. The more we understand the retinal circuitry how it encodes and processes visual information, the greater it could help decide stimulation encoding strategy for retinal prosthesis. Therefore, we examined how retinal ganglion cells (RGCs) in in-vitro retinal preparation act together to encode a visual scene with multielectrode array (MEA). Simultaneous recording of many RGCs with MEA showed that nearby neurons often fired synchronously, with spike delays mostly within 1 ms range. This synchronized firing - narrow correlation - was blocked by gap junction blocker, heptanol, but not by glutamatergic synapse blocker, kynurenic acid. By tracking down all the RGC pairs which showed narrow correlation, we could harvest 40 functional connectivity maps of RGCs which showed the cell cluster firing together. We suggest that finding functional connectivity map would be useful in stimulation encoding strategy for the retinal prosthesis since stimulating the cluster of RGCs would be more efficient than separately stimulating each individual RGC.

참고문헌 (Reference)

1 Humayun MS, "Visual perception in a blind subject with a chronic microelectronic retinal prosthesis" 43 : 2573-2581, 2003

2 Guenther E, "The retina sensor: An in vitro tool to study drug effects on retinal signaling. In: Taketani M, Baudry M, ed, Advances in Network Electrophysiology Using Multi-electrode Arrays" Springer 321-331, 2006

3 Meister M, "The neural code of the retina" 22 : 435-450, 1999

4 Zrenner E, "Subretinal chronic multi-electrode arrays implanted in blind patients" 47 : 1538-, 2006

5 Rizzo JF III, "Shire D. Perceptual efficacy of electrical stimulation of human retina with a microelectrode array during short-term surgical trials" 44 : 5362-5369, 2003

6 Brown SP, "Receptive field microstructure and dendritic geometry of retinal ganglion cells" 27 : 371-383, 2000

7 Lowenstein JI, "Outer retinal degeneration: an electronic retinal prosthesis as a treatment strategy" 122 : 588-596, 2004

8 Caldwell JH, "New properties of rabbit retinal ganglion cells" 276 : 257-276, 1978

9 Meister M, "Multineuronal codes in retinal signaling" 93 : 609-614, 1996

10 Meister M, "Multi-neuronal signals from the retina: acquisition and analysis" 51 : 95-106, 1994

11 DeVries SH, "Mosaic arrangement of ganglion cell receptive fields in rabbit retina" 78 : 2048-2060, 1997

12 Amthor FR, "Morphologies of rabbit retinal ganglion cells with concentric receptive fields" 280 : 72-96, 1989

13 Amthor FR, "Morphologies of rabbit retinal ganglion cells with complex receptive fields" 280 : 97-121, 1989

14 Brivanlou IH, "Mechanisms of concerted firing among retinal ganglion cells" 20 : 527-539, 1998

15 Nakatani K, "Light adaptation in retinal rods of the rabbit and two other nonprimate mammals" 97 : 413-435, 1991

16 Mastronarde DN, "Interactions between ganglion cells in cat retina" 49 : 350-365, 1983

17 Stett A, "Electrical multisite stimulation of isolated chicken retina" 40 : 1785-1795, 2000

18 Margolis DJ, "Different mechanisms generate maintained activity in ON and OFF retinal ganglion cells" 27 : 5994-6005, 2007

19 Arnett D, "Cross-correlation analysis of the maintained discharge of rabbit retinal ganglion cells" 317 : 29-47, 1981

20 DeVries SH, "Correlated firing in rabbit retinal ganglion cells" 81 : 908-920, 1999

21 Meister M, "Concerted signaling by retinal ganglion cells" 270 : 1207-1210, 1995

22 Seo J, "A retinal implant system based on flexible polymer microelectrode array for electrical stimulation In: Visual Prosthesis and Ophthalmic Devices: New Hope in Sight" Humana Press Inc 107-119, 2007

23 Egert U, "A novel organotypic long-term culture of the rat hippocampus on substrate-integrated multielectrode arrays" 2 : 229-242, 1998