This study was designed to observe the absorptive states and metabolism in the body of dietary protein in the course of time after feeding.
The experimental animals, 72 male rats, weighting 270-322 gm were fed with the diets containing isocaloric dif...
This study was designed to observe the absorptive states and metabolism in the body of dietary protein in the course of time after feeding.
The experimental animals, 72 male rats, weighting 270-322 gm were fed with the diets containing isocaloric different level of protein, standard diet group (SD, 16% of protein), high protein diet group (HP, 25% of protein) and low protein diet group (LP, 7% of protein).
The animals were fed with the corresponding diet for 10 days and were sacrificed according to schedule 1 hour, 3 hours and 6 hours after the final feeding. the total nitrogen, nonprotein nitrogen (NPN), amino acid nitrogen, creatinine and uric acid contents in the liver, kidney and serum were determined.
The results obtained are summarized as follows:
1. In the liver, NPN contents of the HP group, creatinine contents of HP or LP group, and uric acid contents of SD group, are higher than any other group.
As for the course of time after feeding, total nitrogen, creatinine and uric acid of each diet group are decreased at 3 hours and are increased at 6 hours.
2. In the kidney, at 1 hour after feeding, total nitrogen contents of SD group, amino aicd nitrogen contents of HP group, creatinine content of HP or LP group and uric acid contents SD or HP group show higher level than any other group.
From 1 hour to 6 hours after feeding, creatinine and uric acid amounts tend to decrease, total nitrogen and amino acid nitrogen levels of LP group are hiher at 3 hours and lower at 6 hours, after feeding.
3. In the serum, at 1 hour after feeding, NPN, amino acid nitrogen and uric acid of HP group are greater in amount and at 3hours, total nitrogen, amino acid nitrogen, creatinine and uric acid contents of LP group are higher, than any other diet group, and at 6 hours, total nitrogen, NPN and amino acid nitrogen contents do not show any significant difference between SD and HP groups, and those of the LP group have lower level.
It is considered from the above results that the diet with 16% protein is good for the metabolism in the body and is also economical, and that the protein in the high protein diet(25%) is not sufficiently utilized. And the metabolism in the body seems most active at 3 hours after feeding, and the low protein diet seems to be utilized in the body faster than the other diets.