<P><B>Summary</B></P><P>Intercellular signal indole and its derivative hydroxyindoles inhibit <I>Escherichia coli</I> biofilm and diminish <I>Pseudomonas aeruginosa</I> virulence. However, indole a...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A107622164
2011
-
SCIE,SCOPUS
학술저널
62-73(12쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P><B>Summary</B></P><P>Intercellular signal indole and its derivative hydroxyindoles inhibit <I>Escherichia coli</I> biofilm and diminish <I>Pseudomonas aeruginosa</I> virulence. However, indole a...
<P><B>Summary</B></P><P>Intercellular signal indole and its derivative hydroxyindoles inhibit <I>Escherichia coli</I> biofilm and diminish <I>Pseudomonas aeruginosa</I> virulence. However, indole and bacterial indole derivatives are unstable in the microbial community because they are quickly degraded by diverse bacterial oxygenases. Hence, this work sought to identify novel, non‐toxic, stable and potent indole derivatives from plant sources for inhibiting the biofilm formation of <I>E. coli</I> O157:H7 and <I>P. aeruginosa</I>. Here, plant auxin 3‐indolylacetonitrile (IAN) was found to inhibit the biofilm formation of both <I>E. coli</I> O157:H7 and <I>P. aeruginosa</I> without affecting its growth. IAN more effectively inhibited biofilms than indole for the two pathogenic bacteria. Additionally, IAN decreased the production of virulence factors including 2‐heptyl‐3‐hydroxy‐4(1<I>H</I>)‐quinolone (PQS), pyocyanin and pyoverdine in <I>P. aeruginosa</I>. DNA microarray analysis indicated that IAN repressed genes involved in curli formation and glycerol metabolism, whereas IAN induced indole‐related genes and prophage genes in <I>E. coli</I> O157:H7. It appeared that IAN inhibited the biofilm formation of <I>E. coli</I> by reducing curli formation and inducing indole production. Also, corroborating phenotypic results of <I>P. aeruginosa</I>, whole‐transcriptomic data showed that IAN repressed virulence‐related genes and motility‐related genes, while IAN induced several small molecule transport genes. Furthermore, unlike bacterial indole derivatives, plant‐originated IAN was stable in the presence of either <I>E. coli</I> or <I>P. aeruginosa</I>. Additionally, indole‐3‐carboxyaldehyde was another natural biofilm inhibitor for both <I>E. coli</I> and <I>P. aeruginosa</I>.</P>