Genetic Stability of the Characters Transformed by Microinjection of Exogenous DNA into Crop Plants = 원예작물에서 외부 DNA 주입에 의하여 유기된 변형형질의 유전적 안정성

다국어 초록 (Multilingual Abstract)

Genetic stability was examined for the second generation(TS-2) progenies of 4 transformants induced by injecting Altari(A) DNA into kungzung(K) radish. All TS-2 plants originated from T3 and T4 had red hypocotyls, suggesting the stable incorporation of the foreign gene and its expression at the second generation. The progenies of these 2 transformants had bigger leaves and heavier roots. However, the segregation of hypocotyl colors of TS-2 plants from T5 and T7 was variable, although the majority still had red hypocotyls. Except the progenises derived from T7, the shapes of cotyledons and the first true leaf, and overall morphology of TS-2 plants as well, were of A types as long as their hypocotyl color was red. For the seedlings to have red hypocotyls, the banding patterns of total suluble proteins and the isozymes of MDH and acid phosphatase should be similar to those of A radish, or at least a mixed type of A and K radish. The later the embryos were removed from the mother plants, and the higher the concn of exogenous DNA up to 10㎍ embryo, the better the differentiation of the explants. The probability to porduce intergeneric hybrids between radish and Chinese cabbage (CC) depended on which was being served as a female. The hybrid plants tended to have mixed banding patterns of total proteins and the isozyme when CC served as a female