Effect of US11 and hDAF Gene for Cytotoxicity Regulate and Transgenic Pigs Breeding

다국어 초록 (Multilingual Abstract)

This study aims to find effect of natural mating and cytotoxicity regulate from US11 and hDAF gene transgenic pig. Piglets that has US11, hDAF or US11&hDAF genes were born from natural mating of female US11 gene transgenic pig and male hDAF gene transgenic pig. Among 10 piglets were born, there are 2 piglets with US11 gene only, 2 piglets with hDAF gene only, 5 piglets with both US11 and hDAF gene, and 1piglets without transgenes. Sex ratio of piglets was 1:1, and transfection rate from natural breeding showed result that only US11 gene was 20%, only hDAF gene was 20%, and both US11 gene and hDAF gene was 50%. In cytotoxicity effect, US11 gene was showed a tendency of reduce human NK cell cytotoxicity in 10:1 ratio. In complement assay, hDAF gene was reduce human complement cytotoxicity in both 1:1 and 10:1 ratio of human complement. hDAF gene was also effected in reduce human NK cell cytotoxicity that showed similar result with US11 gene. Double transgenic piglets fibroblast that has both US11 and hDAF resulted that decrease both human NK cell and complement cytotoxicity compared with wild type cells. In conclusion, Natural mating was effected for US11 and hDAF gene transmitted into offsping piglets, and US11 and hDAF gene were expected to regulate xenograft rejections in transgenic tissues.