Characterization of Cell Viability in Phaeocystis globosa Cultures Exposed to Marine Algicidal Bacteria

다국어 초록 (Multilingual Abstract)

The bloom of Phaeocystis globosa has occurredfrequently in the southern coastal areas of China in recentyears, which has led to substantial economic losses. Thisstudy investigated the effects of culture broth of strains Y1and Y4 isolated from algal blooms in Zhuhai, China onphysiological characteristics and cell viability of P. globosa.
The increase in the levels of reactive oxygen species (ROS)in P. globosa cells exposed to strains Y1 and Y4 culturebroth were detected, indicating that the algal cells sufferedfrom oxidative damage. The surplus ROS induced theincrease of malondialdehyde (MDA) contents and theactivities of antioxidant enzymes, including superoxidedismutase (SOD) and catalase (CAT). The decrease inprotein content indicated that strains Y1 and Y4 culturebroth inhibited cell growth. The contents of pigmentsdecreased after 96 h treatment, indicated that oxidative stressdestroyed pigment synthesis. Furthermore, flow cytometrycoupled with the propidium iodide stain and chlorophyllauto-fluorescence was used to investigate cell viability.
Results showed that chlorophyll fluorescence intensitiesand cell integrity decreased with time of exposure, whichdemonstrated that strains Y1 and Y4 culture broth couldchange membrane permeability and resulted in the loss ofthese photosynthetic pigments. The isolated strains wereidentified as Bacillus sp. by culture morphology, biochemicalreactions, and homology research based on 16S rDNA.
Overall, these findings suggested that oxidative stress causedfrom Bacillus sp. potentially destroyed pigment synthesisand cell membrane integrity, and ultimately led to the lysisof the algal cells.

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