Physiological and genetic characteristics of intestinal bacteria isolated from aquatic and terrestrial organisms in Jeju

목차 (Table of Contents)

• ABSTRACT 1
• CHAPTER Ⅰ. INTRODUCTION 3
• CHAPTER Ⅱ. Characterization of fermentation bacteria isolated from horse and wild roe deer feces in Jeju Island using the culture-dependent method
• 2.1 INTRODUCTION 4
• 2.2 MATERIALS AND METHODS 7
• 2.2.1 Sample Collection and bacterial strain isolation 7
• 2.2.2 Identification of morphological characteristics 9
• 2.2.3 Confirmation of genetic phylogenetic characteristics 10
• 2.2.4 Confirmation of fermentation ability 11
• 2.3 RESULTS 12
• 2.3.1 Confirmation of genetic and morphological characteristics of isolated cultured strains 12
• 2.3.2 Confirmation of acid production and fermentation ability 22
• 2.4 DISCUSSION 25
• CHAPTER Ⅲ. Kinetic, Genomic, and Physiological Analysis Reveals Diversity in the Ecological Adaptation, and Metabolic Potential of a Novel Species, Brachybacterium equifaecis
• 3.1 INTRODUCTION 28
• 3.2 MATERIALS AND METHODS 30
• 3.2.1 Isolation and Morphological Characterization 30
• 3.2.2 Phylogenetic Analysis for Strain Identification 31
• 3.2.3 Physiological and Biochemical Characterization 32
• 3.2.4 Chemotaxonomic Analyses 34
• 3.2.5 Lactic acid product of Fermentation experiment 35
• 3.2.6 Genome Sequencing and Analyses 36
• 3.2.7 Determining Kinetic Properties 38
• 3.2.8 Nucleotide and Whole-genome Sequence Accession Numbers 39
• 3.3 RESULTS 40
• 3.3.1 Morphological, Physiological, and Biochemical Characterization 40
• 3.3.2 Lactic acid Production by Fermentation 48
• 3.3.3 Identification of Novel Strain JHP9 and Phylogenetic Clustering 50
• 3.3.4 Genome Functional Annotation 58
• 3.3.5 Substrate and Oxygen Affinity 66
• 3.4 DISCUSSION 69
• 3.5 Description of Brachybacterium equifaecis JHP9 sp. nov. 77
• CHAPTER Ⅳ. Granulicatella seriolae sp. nov., A Novel Facultative Anaerobe Isolated from Yellowtail Marine Fish
• 4.1 INTRODUCTION 79
• 4.2 MATERIALS AND METHODS 80
• 4.2.1 Isolation of strain S8 80
• 4.2.2 Morphological, Physiological, and Biochemical Characterization 81
• 4.2.3 Chemotaxonomic Analysis 82
• 4.2.4 Species Identification 83
• 4.2.5 Draft Genome Sequence Analysis 84
• 4.3 Result and Discussion 85
• 4.3.1 Strain S8 Morphology and Physiology 85
• 4.3.2 Species Identification and Genomic Features 91
• 4.3.3 Description of Granulicatella seriolae sp. nov. 95
• CHAPTER Ⅴ. CONCLUSION 96
• SUPLYMENTERY FILE 1 98
• SUPLYMENTERY FILE 2 103
• ABSTRACT (KOREAN Ver.) 156
• REFERENCES 158