자궁상피내암종 및 침윤성 편평상피암에서의 In Situ Hybridization 에 의한 Human Papillomavirus DNA 의 검색 = Detection of Human Papillomavirus DNA by In Situ Hybridization using Biotinylated DNA Probes in Cervieal Intraepithelial Neoplasias and Squamous Cell Carcinomas

다국어 초록 (Multilingual Abstract)

The authors examined 9 condylomas, 26 cervical intraepithelial neoplasms(CIN) and 22 invasive squamous cell carcinomas for the presence of human papillomavirus(HPV) DNA sequences by DNA-DNA in situ hybridization. In situ hybridization revealed target HPV DNA sequences mostly in the nuclei of the superficial cells from epithelium which contained either maturation or koilocytotic atypias. With the use of biotinylated HPV DNA probes 6/11, 16/18 and 31/33/35, 42 of the 57(73.7%) were positive with any HPV DNA mixed probes, 13 of which were positive with HPV-6/11, 23 with HPV-16/18, 32 with HPV-31/33/35 and 18 with two or more mixed probes. HPV-31/33/35 was the most prevalent in CIN and invasive squamous cell carcinomas, followed by HPV-16/18. The incidence of HPV DNA increased from 66.7% to 86.496 with increasing severity of the lesions from condylomas to invasive squamous cell carcinomas. Flat condyloma was most frequently accompanied by CIN.